Detailed information of TA system TA196177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 525076..525806
• Replicon: chromosome
• Accession: NZ_CP071320
• Organism: Serratia ureilytica strain T6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: J0X03_RS02425
• Protein ID: WP_004937033.1
• Coordinates: 525495..525806 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J0X03_RS02420
• Protein ID: WP_033644489.1
• Coordinates: 525076..525498 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0X03_RS02405	521059..521799	+	741	WP_160204647.1	KDGP aldolase family protein	-
J0X03_RS02410	521887..523101	+	1215	WP_160204646.1	lactonase family protein	-
J0X03_RS02415	523153..525069	+	1917	WP_033644486.1	BglG family transcription antiterminator	-
J0X03_RS02420	525076..525498	-	423	WP_033644489.1	helix-turn-helix domain-containing protein	Antitoxin
J0X03_RS02425	525495..525806	-	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
J0X03_RS02430	526101..526373	-	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
J0X03_RS02435	526370..527224	-	855	WP_015379177.1	RNase adapter RapZ	-
J0X03_RS02440	527352..527813	-	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
J0X03_RS02445	527969..528256	-	288	WP_004937038.1	ribosome hibernation promoting factor	-
J0X03_RS02450	528280..529713	-	1434	WP_043141961.1	RNA polymerase factor sigma-54	-
J0X03_RS02455	529766..530491	-	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T196177 WP_004937033.1 NZ_CP071320:c525806-525495 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T196177 NZ_CP095022:1689861-1690178 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT196177 WP_033644489.1 NZ_CP071320:c525498-525076 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT196177 NZ_CP095022:1689562-1689864 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63