Detailed information of TA system TA196172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 48209..48794
• Replicon: plasmid pGXNN15
• Accession: NZ_CP071318
• Organism: Acinetobacter indicus strain GXNN15X4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: J0W32_RS14285
• Protein ID: WP_000897307.1
• Coordinates: 48474..48794 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J0W32_RS14280
• Protein ID: WP_000369781.1
• Coordinates: 48209..48481 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0W32_RS14265	44500..45111	+	612	WP_015060246.1	recombinase family protein	-
J0W32_RS14270	45301..46185	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
J0W32_RS14275	46241..47716	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
J0W32_RS14280	48209..48481	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
J0W32_RS14285	48474..48794	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J0W32_RS14290	49152..50720	+	1569	WP_159124549.1	ATP-binding cassette domain-containing protein	-
J0W32_RS14295	50933..51076	-	144	WP_001125248.1	hypothetical protein	-
J0W32_RS14300	51089..51641	-	553	Protein_49	helix-turn-helix domain-containing protein	-
J0W32_RS14305	51634..52014	-	381	Protein_50	replication initiation protein	-
J0W32_RS14310	52069..52367	+	299	Protein_51	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / dfrA16 / blaCARB-2 / aadA8b / sul3 / tet(39) / mph(E) / msr(E)	-	1..87911	87911
	-	inside	IScluster/Tn	floR / dfrA16 / blaCARB-2 / aadA8b / sul3 / tet(39) / mph(E) / msr(E)	-	25205..59513	34308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T196172 WP_000897307.1 NZ_CP071318:c48794-48474 [Acinetobacter indicus]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T196172 NZ_CP095022:1238262-1238636 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT196172 WP_000369781.1 NZ_CP071318:c48481-48209 [Acinetobacter indicus]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT196172 NZ_CP095022:1238068-1238265 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3