Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 34610..35258 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag J0G25_RS00170 Protein ID WP_016151211.1
Coordinates 34610..34972 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag J0G25_RS00175 Protein ID WP_003840660.1
Coordinates 34959..35258 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS00155 (31069) 31069..32397 + 1329 WP_003023927.1 MFS transporter -
J0G25_RS00160 (32540) 32540..33931 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
J0G25_RS00165 (34057) 34057..34509 + 453 WP_003023933.1 DUF1198 family protein -
J0G25_RS00170 (34610) 34610..34972 + 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J0G25_RS00175 (34959) 34959..35258 + 300 WP_003840660.1 XRE family transcriptional regulator Antitoxin
J0G25_RS00180 (35377) 35377..36570 + 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
J0G25_RS00185 (36619) 36619..38001 - 1383 WP_016151210.1 glycoside hydrolase family 1 protein -
J0G25_RS00190 (38096) 38096..38389 - 294 WP_003840665.1 YicS family protein -
J0G25_RS00195 (38520) 38520..38936 + 417 WP_003023951.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T196116 WP_016151211.1 NZ_CP071265:34610-34972 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T196116 NZ_CP095021:c1390239-1389946 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT196116 WP_003840660.1 NZ_CP071265:34959-35258 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT196116 NZ_CP095021:c1390505-1390236 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References