Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1738586..1739313 Replicon chromosome
Accession NZ_CP071263
Organism Escherichia coli strain MEI003

Toxin (Protein)


Gene name higB Uniprot ID D6JE58
Locus tag J0G23_RS08535 Protein ID WP_000547557.1
Coordinates 1739002..1739313 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1M2G9D6
Locus tag J0G23_RS08530 Protein ID WP_001600394.1
Coordinates 1738586..1739005 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G23_RS08510 (1733671) 1733671..1734198 - 528 WP_001078777.1 electron transport protein HydN -
J0G23_RS08515 (1734347) 1734347..1735357 - 1011 WP_024229842.1 DNA-binding transcriptional regulator AscG -
J0G23_RS08520 (1735617) 1735617..1737074 + 1458 WP_001107867.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
J0G23_RS08525 (1737083) 1737083..1738507 + 1425 WP_024229843.1 6-phospho-beta-glucosidase AscB -
J0G23_RS08530 (1738586) 1738586..1739005 - 420 WP_001600394.1 helix-turn-helix domain-containing protein Antitoxin
J0G23_RS08535 (1739002) 1739002..1739313 - 312 WP_000547557.1 type II toxin-antitoxin system HigB family toxin Toxin
J0G23_RS08540 (1739480) 1739480..1739950 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
J0G23_RS08545 (1739943) 1739943..1740353 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
J0G23_RS08550 (1740350) 1740350..1741117 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
J0G23_RS08555 (1741117) 1741117..1741659 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
J0G23_RS08560 (1741669) 1741669..1743378 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12467.20 Da        Isoelectric Point: 9.5262

>T196095 WP_000547557.1 NZ_CP071263:c1739313-1739002 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T196095 NZ_CP095021:361895-362197 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15389.40 Da        Isoelectric Point: 4.4596

>AT196095 WP_001600394.1 NZ_CP071263:c1739005-1738586 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT196095 NZ_CP095021:361671-361898 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D6JE58

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2G9D6

References