Detailed information of TA system TA196079

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 141851..142076
• Replicon: chromosome
• Accession: NZ_CP071263
• Organism: Escherichia coli strain MEI003

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: J0G23_RS00755
• Protein ID: WP_000813266.1
• Coordinates: 141921..142076 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	141851..141909 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G23_RS00720	137364..138110	+	747	WP_000788766.1	ATP-binding protein	-
J0G23_RS00725	138133..138894	+	762	WP_024229933.1	DUF1627 domain-containing protein	-
J0G23_RS00730	138902..139318	+	417	WP_024229932.1	DUF977 family protein	-
J0G23_RS00735	139315..139578	+	264	WP_024229931.1	hypothetical protein	-
J0G23_RS00740	139565..139876	+	312	WP_032320575.1	hypothetical protein	-
J0G23_RS00745	140010..140564	-	555	WP_024229929.1	hypothetical protein	-
J0G23_RS00750	140561..141493	-	933	WP_024229928.1	hypothetical protein	-
-	141851..141909	-	59	-	-	Antitoxin
J0G23_RS00755	141921..142076	+	156	WP_000813266.1	Hok/Gef family protein	Toxin
J0G23_RS00760	142545..143144	+	600	WP_000940314.1	DUF1367 family protein	-
J0G23_RS00765	143144..143434	+	291	WP_000228031.1	DUF1364 domain-containing protein	-
J0G23_RS00770	143431..143973	+	543	WP_000640136.1	DUF1133 family protein	-
J0G23_RS25725	145102..145227	-	126	WP_001298832.1	hypothetical protein	-
J0G23_RS00785	145260..145475	+	216	WP_000839599.1	phage lysis protein EssD	-
J0G23_RS00790	145475..145972	+	498	WP_001135280.1	lysozyme RrrD	-
J0G23_RS00795	146189..146374	+	186	WP_001228696.1	prophage lysis lipoprotein RzoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	126653..176487	49834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5813.99 Da
• Isoelectric Point: 7.7951

>T196079 WP_000813266.1 NZ_CP071263:141921-142076 [Escherichia coli]
MKQQKAMLIAMIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESRE

Nucleotide

• Length: 156 bp

>T196079 NZ_CP095020:c2864752-2864357 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT196079 NZ_CP071263:c141909-141851 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin