Detailed information of TA system TA196075

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 128209..128580
• Replicon: chromosome
• Accession: NZ_CP071263
• Organism: Escherichia coli strain MEI003

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: J0G23_RS00660
• Protein ID: WP_021500490.1
• Coordinates: 128386..128580 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	128209..128387 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G23_RS25715 (123961)	123961..124134	+	174	WP_001296046.1	protein YnaL	-
J0G23_RS00635 (124164)	124164..125537	+	1374	WP_001599773.1	ATP-dependent RNA helicase DbpA	-
J0G23_RS00640 (125666)	125666..126601	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
J0G23_RS00645 (126653)	126653..127888	-	1236	WP_000040838.1	site-specific integrase	-
J0G23_RS00650 (127890)	127890..128105	-	216	WP_000079604.1	excisionase XisR	-
- (128209)	128209..128387	+	179	NuclAT_0	-	Antitoxin
- (128209)	128209..128387	+	179	NuclAT_0	-	Antitoxin
- (128209)	128209..128387	+	179	NuclAT_0	-	Antitoxin
- (128209)	128209..128387	+	179	NuclAT_0	-	Antitoxin
J0G23_RS00655 (128205)	128205..128393	-	189	WP_001298826.1	DUF1187 family protein	-
J0G23_RS00660 (128386)	128386..128580	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
J0G23_RS00665 (128644)	128644..129696	-	1053	WP_001004422.1	RecT family recombinase	-
J0G23_RS00670 (129708)	129708..132779	-	3072	WP_024229934.1	exodeoxyribonuclease VIII	-
J0G23_RS00675 (132881)	132881..133156	-	276	WP_001314664.1	hypothetical protein	-
J0G23_RS00680 (133231)	133231..133401	-	171	WP_001516462.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	126653..176487	49834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T196075 WP_021500490.1 NZ_CP071263:c128580-128386 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T196075 NZ_CP095020:2802947-2803372 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT196075 NZ_CP071263:128209-128387 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin