Detailed information of TA system TA196056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4960096..4960698
• Replicon: chromosome
• Accession: NZ_CP071259
• Organism: Escherichia coli strain MEI005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: J0G24_RS23895
• Protein ID: WP_000897305.1
• Coordinates: 4960387..4960698 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J0G24_RS23890
• Protein ID: WP_000356397.1
• Coordinates: 4960096..4960386 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G24_RS23865 (4956022)	4956022..4956924	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
J0G24_RS23870 (4956921)	4956921..4957556	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
J0G24_RS23875 (4957553)	4957553..4958482	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
J0G24_RS23880 (4958812)	4958812..4959054	-	243	WP_001087409.1	protein YiiF	-
J0G24_RS23885 (4959273)	4959273..4959491	-	219	WP_001295676.1	CopG family transcriptional regulator	-
J0G24_RS23890 (4960096)	4960096..4960386	-	291	WP_000356397.1	NadS family protein	Antitoxin
J0G24_RS23895 (4960387)	4960387..4960698	-	312	WP_000897305.1	hypothetical protein	Toxin
J0G24_RS23900 (4960927)	4960927..4961835	+	909	WP_001162704.1	alpha/beta hydrolase	-
J0G24_RS23905 (4961899)	4961899..4962840	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
J0G24_RS23910 (4962885)	4962885..4963322	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
J0G24_RS23915 (4963319)	4963319..4964191	-	873	WP_000920762.1	virulence factor BrkB family protein	-
J0G24_RS23920 (4964185)	4964185..4964784	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T196056 WP_000897305.1 NZ_CP071259:c4960698-4960387 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T196056 NZ_CP095020:1238453-1238827 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT196056 WP_000356397.1 NZ_CP071259:c4960386-4960096 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT196056 NZ_CP095020:1238259-1238456 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7