Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3977061..3977602 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag J0G24_RS19405 Protein ID WP_000615976.1
Coordinates 3977324..3977602 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag J0G24_RS19400 Protein ID WP_000729704.1
Coordinates 3977061..3977321 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS19375 (3972844) 3972844..3973629 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
J0G24_RS19380 (3973601) 3973601..3975313 + 1713 Protein_3805 flagellar biosynthesis protein FlhA -
J0G24_RS19385 (3975418) 3975418..3975696 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
J0G24_RS19390 (3975689) 3975689..3976045 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
J0G24_RS19395 (3976102) 3976102..3976875 - 774 WP_000543899.1 C40 family peptidase -
J0G24_RS19400 (3977061) 3977061..3977321 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J0G24_RS19405 (3977324) 3977324..3977602 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J0G24_RS19410 (3977758) 3977758..3978498 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
J0G24_RS19415 (3978469) 3978469..3979236 - 768 WP_000333380.1 class II glutamine amidotransferase -
J0G24_RS19420 (3979442) 3979442..3980020 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T196052 WP_000615976.1 NZ_CP071259:3977324-3977602 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T196052 NZ_CP095020:c754816-754379 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT196052 WP_000729704.1 NZ_CP071259:3977061-3977321 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT196052 NZ_CP095020:c755067-754813 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References