Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 703877..704676 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag J0G24_RS03505 Protein ID WP_000347267.1
Coordinates 703877..704341 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J0G24_RS03510 Protein ID WP_001307405.1
Coordinates 704341..704676 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS03475 (698878) 698878..699312 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J0G24_RS03480 (699330) 699330..700208 - 879 WP_001380079.1 PTS N-acetylgalactosamine transporter subunit IID -
J0G24_RS03485 (700198) 700198..700977 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
J0G24_RS03490 (700988) 700988..701461 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J0G24_RS03495 (701484) 701484..702764 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J0G24_RS03500 (703013) 703013..703822 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J0G24_RS03505 (703877) 703877..704341 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J0G24_RS03510 (704341) 704341..704676 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J0G24_RS03515 (704825) 704825..706396 - 1572 WP_001273741.1 galactarate dehydratase -
J0G24_RS03520 (706771) 706771..708105 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J0G24_RS03525 (708121) 708121..708891 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T196033 WP_000347267.1 NZ_CP071259:c704341-703877 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T196033 NZ_CP095011:c3065539-3065285 [Streptomyces sp. 2P-4]
GTGAAAGTTGTCTTCTCGGGCCGGGCATGGGAGCAGTACACGTACTGGGCAACTGCCGACCCGAAGATCCTCAAGCGGAT
CAACCGCTTGATCGGCGAAATCCGGCGTACACCCTTCGAAGGCACTGGGAAGCCGGAGCCGCTCAAGGAGAACCTGTCGG
GCTGGTGGTCTCGGCGGATCACCGACGAGCACCGGCTGGTGTACCGGATCCAGGACCAAGCAGTGGAGGTCGCACAGGTC
CGGTACCACTACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT196033 WP_001307405.1 NZ_CP071259:c704676-704341 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT196033 NZ_CP095011:c3065817-3065536 [Streptomyces sp. 2P-4]
ATGTCGATGTCAGCCAATGAGGCCCGTCAGCGCTTCTATCCGCTGATCCGGCAGGTCAACGAAGACCACGTGCCCATAGA
GGTCACGTCCCGGGAGCACGGTGACGTGGTGATCATGTCGGCTGAAGACTTCCGCTCTTGGCAGGAAACGGTCTATCTCC
TGCGCTCGCCGCGAAACGCGCAGATCCTGATGGAATCGATCGCAGAGCTGGATGCCGGACAAGGCCAGGCCAGAGAGCTG
ATCAGCGTCGACGACGAGGAAGAAGCCGGGTCCGCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References