Detailed information of TA system TA195999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 712208..712784
• Replicon: chromosome
• Accession: NZ_CP071246
• Organism: Corynebacterium sp. CNCTC7651

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: JZY91_RS03505
• Protein ID: WP_234948583.1
• Coordinates: 712208..712489 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JZY91_RS03510
• Protein ID: WP_234948584.1
• Coordinates: 712524..712784 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZY91_RS03480	709129..709443	+	315	WP_012359882.1	hypothetical protein	-
JZY91_RS03485 (JZY91_03475)	709531..709881	-	351	WP_234948581.1	hypothetical protein	-
JZY91_RS03490 (JZY91_03480)	709878..711299	-	1422	WP_234948582.1	mercury(II) reductase	-
JZY91_RS03495 (JZY91_03485)	711398..711787	+	390	WP_005290994.1	heavy metal-responsive transcriptional regulator	-
JZY91_RS03500 (JZY91_03490)	711831..711959	-	129	WP_005293248.1	monooxygenase	-
JZY91_RS03505 (JZY91_03495)	712208..712489	+	282	WP_234948583.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JZY91_RS03510 (JZY91_03500)	712524..712784	+	261	WP_234948584.1	HigA family addiction module antitoxin	Antitoxin
JZY91_RS03515 (JZY91_03505)	712975..713078	+	104	Protein_683	GNAT family N-acetyltransferase	-
JZY91_RS03520 (JZY91_03510)	713115..714215	+	1101	WP_234948585.1	Dyp-type peroxidase	-
JZY91_RS11700	714185..714484	-	300	WP_255695752.1	DUF488 domain-containing protein	-
JZY91_RS03525	714430..714783	-	354	WP_234948586.1	DUF488 family protein	-
JZY91_RS03530 (JZY91_03520)	714889..715383	-	495	WP_234948588.1	DUF1707 domain-containing protein	-
JZY91_RS03535 (JZY91_03525)	715435..716208	+	774	WP_234948590.1	MerR family transcriptional regulator	-
JZY91_RS03540 (JZY91_03530)	716424..716774	-	351	WP_234948591.1	VOC family protein	-
JZY91_RS03545 (JZY91_03535)	716808..717251	-	444	WP_234948592.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-82)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10902.43 Da
• Isoelectric Point: 10.0866

>T195999 WP_234948583.1 NZ_CP071246:712208-712489 [Corynebacterium sp. CNCTC7651]
MIQSFADKDTERLWNRERVRSIDPRIHSVALRKLRILGSAQSLEDLRIPPGNRLEALKGDRQGQHSIRINGQWRICFRWT
VAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T195999 NZ_CP094992:45412-45696 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAGAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9645.08 Da
• Isoelectric Point: 5.0495

>AT195999 WP_234948584.1 NZ_CP071246:712524-712784 [Corynebacterium sp. CNCTC7651]
MEDFLNEMGITQHKLAVSIGVPPRRINEIAHGKRAITADTALRLAKFFGVSPQFWLGLQTQYDLDEAEDRILAEIEAIQP
LQVARA

Nucleotide

• Length: 261 bp

>AT195999 NZ_CP094992:45174-45422 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA