Detailed information of TA system TA195996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4534593..4535323
• Replicon: chromosome
• Accession: NZ_CP071244
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S37_jyu2015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: J0F95_RS21690
• Protein ID: WP_004937033.1
• Coordinates: 4534593..4534904 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J0F95_RS21695
• Protein ID: WP_015379178.1
• Coordinates: 4534901..4535323 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F95_RS21660	4529906..4530631	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
J0F95_RS21665	4530684..4532117	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
J0F95_RS21670	4532141..4532428	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
J0F95_RS21675	4532584..4533045	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
J0F95_RS21680	4533175..4534029	+	855	WP_015379177.1	RNase adapter RapZ	-
J0F95_RS21685	4534026..4534298	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
J0F95_RS21690	4534593..4534904	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
J0F95_RS21695	4534901..4535323	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
J0F95_RS21700	4535327..4537243	-	1917	WP_033639303.1	BglG family transcription antiterminator	-
J0F95_RS21705	4537295..4538509	-	1215	WP_033639302.1	lactonase family protein	-
J0F95_RS21710	4538786..4539526	-	741	WP_016930215.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T195996 WP_004937033.1 NZ_CP071244:4534593-4534904 [Serratia marcescens subsp. marcescens ATCC 13880]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T195996 NZ_CP094991:c4791364-4791080 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT195996 WP_015379178.1 NZ_CP071244:4534901-4535323 [Serratia marcescens subsp. marcescens ATCC 13880]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT195996 NZ_CP094991:c4791596-4791354 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63