Detailed information of TA system TA195548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4534612..4535342
• Replicon: chromosome
• Accession: NZ_CP071188
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S94_jyu2015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: J0F99_RS21690
• Protein ID: WP_004937033.1
• Coordinates: 4534612..4534923 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J0F99_RS21695
• Protein ID: WP_015379178.1
• Coordinates: 4534920..4535342 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F99_RS21660	4529925..4530650	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
J0F99_RS21665	4530703..4532136	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
J0F99_RS21670	4532160..4532447	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
J0F99_RS21675	4532603..4533064	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
J0F99_RS21680	4533194..4534048	+	855	WP_015379177.1	RNase adapter RapZ	-
J0F99_RS21685	4534045..4534317	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
J0F99_RS21690	4534612..4534923	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
J0F99_RS21695	4534920..4535342	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
J0F99_RS21700	4535346..4537262	-	1917	WP_033639303.1	BglG family transcription antiterminator	-
J0F99_RS21705	4537314..4538528	-	1215	WP_033639302.1	lactonase family protein	-
J0F99_RS21710	4538805..4539545	-	741	WP_016930215.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T195548 WP_004937033.1 NZ_CP071188:4534612-4534923 [Serratia marcescens subsp. marcescens ATCC 13880]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T195548 NZ_CP094758:c1924488-1924336 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT195548 WP_015379178.1 NZ_CP071188:4534920-4535342 [Serratia marcescens subsp. marcescens ATCC 13880]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT195548 NZ_CP094758:c1925112-1924513 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATGGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63