Detailed information of TA system TA195516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1152212..1152861
• Replicon: chromosome
• Accession: NZ_CP071178
• Organism: Enterobacter kobei strain CDL29

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0PLQ3
• Locus tag: JZY03_RS05360
• Protein ID: WP_014882864.1
• Coordinates: 1152499..1152861 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JZY03_RS05355
• Protein ID: WP_023338139.1
• Coordinates: 1152212..1152511 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZY03_RS05345 (JZY03_05330)	1148637..1150610	+	1974	WP_023338138.1	PhoX family phosphatase	-
JZY03_RS05350 (JZY03_05335)	1150713..1152101	+	1389	WP_014882862.1	phenylalanine transporter	-
JZY03_RS05355 (JZY03_05340)	1152212..1152511	-	300	WP_023338139.1	helix-turn-helix transcriptional regulator	Antitoxin
JZY03_RS05360 (JZY03_05345)	1152499..1152861	-	363	WP_014882864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JZY03_RS05365 (JZY03_05350)	1153067..1154302	+	1236	WP_023332295.1	MFS transporter	-
JZY03_RS05370 (JZY03_05355)	1154318..1155343	+	1026	WP_023338140.1	sugar phosphate isomerase/epimerase	-
JZY03_RS05375 (JZY03_05360)	1155336..1156478	+	1143	WP_023338141.1	Gfo/Idh/MocA family oxidoreductase	-
JZY03_RS05380 (JZY03_05365)	1156554..1157525	+	972	WP_014882868.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13952.92 Da
• Isoelectric Point: 6.7114

>T195516 WP_014882864.1 NZ_CP071178:c1152861-1152499 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T195516 NZ_CP094752:356780-356956 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10876.61 Da
• Isoelectric Point: 9.8627

>AT195516 WP_023338139.1 NZ_CP071178:c1152511-1152212 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRISFQI

Nucleotide

• Length: 300 bp

>AT195516 NZ_CP094752:c357118-356944 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGTGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A2J0PLQ3