Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31365..32095 Replicon chromosome
Accession NZ_CP071178
Organism Enterobacter kobei strain CDL29

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag JZY03_RS00150 Protein ID WP_045134487.1
Coordinates 31365..31679 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JZY03_RS00155 Protein ID WP_023331687.1
Coordinates 31676..32095 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZY03_RS00130 (JZY03_00130) 27030..28118 + 1089 WP_032635252.1 cellulase family glycosylhydrolase -
JZY03_RS00135 (JZY03_00135) 28021..29205 - 1185 WP_032637792.1 multidrug efflux MFS transporter EmrD -
JZY03_RS00140 (JZY03_00140) 29381..30214 - 834 WP_045134488.1 EamA family transporter -
JZY03_RS00145 (JZY03_00145) 30277..30723 - 447 WP_014881927.1 GNAT family N-acetyltransferase -
JZY03_RS00150 (JZY03_00155) 31365..31679 + 315 WP_045134487.1 type II toxin-antitoxin system HigB family toxin Toxin
JZY03_RS00155 (JZY03_00160) 31676..32095 + 420 WP_023331687.1 helix-turn-helix domain-containing protein Antitoxin
JZY03_RS00160 (JZY03_00165) 32245..32343 + 99 WP_023331688.1 ilvB operon leader peptide IvbL -
JZY03_RS00165 (JZY03_00170) 32450..34138 + 1689 WP_023331689.1 acetolactate synthase large subunit -
JZY03_RS00170 (JZY03_00175) 34142..34429 + 288 WP_010426528.1 acetolactate synthase small subunit -
JZY03_RS00175 (JZY03_00180) 34512..35105 + 594 WP_014881931.1 transcriptional regulator UhpA -
JZY03_RS00180 (JZY03_00185) 35102..36607 + 1506 WP_045134486.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12455.66 Da        Isoelectric Point: 10.3843

>T195513 WP_045134487.1 NZ_CP071178:31365-31679 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNKLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T195513 NZ_CP094750:c2138364-2138155 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15561.18 Da        Isoelectric Point: 5.6761

>AT195513 WP_023331687.1 NZ_CP071178:31676-32095 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT195513 NZ_CP094750:c2138669-2138376 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References