Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2266801..2267720 Replicon chromosome
Accession NZ_CP071127
Organism Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JY387_RS10550 Protein ID WP_003900449.1
Coordinates 2267115..2267720 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JY387_RS10545 Protein ID WP_003410124.1
Coordinates 2266801..2267106 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY387_RS10515 2261812..2263068 - 1257 WP_003410095.1 HNH endonuclease -
JY387_RS10520 2263422..2263997 + 576 WP_003410100.1 hypothetical protein -
JY387_RS10525 2263994..2265034 + 1041 WP_206280167.1 ImmA/IrrE family metallo-endopeptidase -
JY387_RS10530 2265276..2265995 + 720 WP_003410108.1 DUF433 domain-containing protein -
JY387_RS10535 2265985..2266401 + 417 WP_003410114.1 hypothetical protein -
JY387_RS10540 2266417..2266716 - 300 WP_206280168.1 hypothetical protein -
JY387_RS10545 2266801..2267106 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JY387_RS10550 2267115..2267720 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JY387_RS10555 2267745..2268104 - 360 WP_003410131.1 hypothetical protein -
JY387_RS10560 2268264..2268716 - 453 Protein_2087 hypothetical protein -
JY387_RS10565 2268719..2270236 - 1518 Protein_2088 DEAD/DEAH box helicase -
JY387_RS10570 2270746..2271744 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T195366 WP_003900449.1 NZ_CP071127:c2267720-2267115 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T195366 NZ_CP094663:c1666373-1666269 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT195366 WP_003410124.1 NZ_CP071127:c2267106-2266801 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT195366 NZ_CP094663:1666176-1666214 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References