Detailed information of TA system TA195325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2342141..2342658
• Replicon: chromosome
• Accession: NZ_CP071104
• Organism: Staphylococcus aureus strain PS/BAC/317/16/W

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A170NWK0
• Locus tag: JYA73_RS11380
• Protein ID: WP_000251928.1
• Coordinates: 2342141..2342407 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A166LMC5
• Locus tag: JYA73_RS11385
• Protein ID: WP_000584497.1
• Coordinates: 2342407..2342658 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA73_RS11355 (JYA73_002274)	2337349..2337885	-	537	WP_000024292.1	GNAT family N-acetyltransferase	-
JYA73_RS11360 (JYA73_002275)	2338353..2339177	-	825	WP_000594640.1	formate/nitrite transporter family protein	-
JYA73_RS11365 (JYA73_002276)	2339380..2339562	-	183	WP_000079140.1	hypothetical protein	-
JYA73_RS11370 (JYA73_002277)	2339648..2340115	-	468	WP_000153340.1	SRPBCC domain-containing protein	-
JYA73_RS11375 (JYA73_002278)	2340301..2341848	-	1548	WP_000727771.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
JYA73_RS11380 (JYA73_002279)	2342141..2342407	-	267	WP_000251928.1	Txe/YoeB family addiction module toxin	Toxin
JYA73_RS11385 (JYA73_002280)	2342407..2342658	-	252	WP_000584497.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JYA73_RS11390 (JYA73_002281)	2342822..2342922	-	101	Protein_2211	hypothetical protein	-
JYA73_RS11395 (JYA73_002282)	2342929..2343528	-	600	WP_000162804.1	DsbA family protein	-
JYA73_RS11400 (JYA73_002283)	2343547..2343909	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
JYA73_RS11405 (JYA73_002284)	2344164..2345414	+	1251	WP_001012237.1	FemA/FemB family glycyltransferase FmhA	-
JYA73_RS11410 (JYA73_002285)	2345511..2346242	-	732	WP_000615467.1	amino acid ABC transporter ATP-binding protein	-
JYA73_RS11415 (JYA73_002286)	2346239..2346958	-	720	WP_000479573.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.06 Da
• Isoelectric Point: 10.1628

>T195325 WP_000251928.1 NZ_CP071104:c2342407-2342141 [Staphylococcus aureus]
MVRLNITFSPQAFEDYKYFQQNDKKMVKKINELIKSIDRNGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T195325 NZ_CP094526:7854476-7854829 [Bradyrhizobium sp. ISRA464]
TTGGCAACGAAGTCTGGTAACTACTGTCCCGAGGCCGGCGACTTCGTCTGGATCGATCTTGATCCCACGATCGGCCACGA
ACAAAGCGGCCGACGACCGGCCATCGTATTATCGCCGCACAACTACAATGCCGCAGCCGGCCTTTGCGTGATCTGCCCAA
TCACAAGCAGCGTACGCGACTATCCCTTCGAGGCCAAGCTGCCCGACGGCGCCAGCTTTCACGGCGTCGTGCTCGCCGAC
CAACCCCGCAGCATCTCGTGGGAGAAGCGACCGATCCAGCATGCCGGACGAGCGCCTGACGTCCTGCTCACCGAAGTCCG
TGAGCGTTTGGCCGCGTTGCTCGGAATCGACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9401.54 Da
• Isoelectric Point: 4.3407

>AT195325 WP_000584497.1 NZ_CP071104:c2342658-2342407 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDIVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTVTKD
IDV

Nucleotide

• Length: 252 bp

>AT195325 NZ_CP094526:7854241-7854495 [Bradyrhizobium sp. ISRA464]
ATGAAGGTAGCATTTCAGAAGTGGGGCAACAGCCTGGCACTGAGAGTTCCAAAAGCATTCGCCGATGAAATCGGTGCCAG
CGACGGCAAGGCCGCCGAAATGACCGTGAGCGACGGCAAACTGGTGATCGAGATCGCGCGCTCGCCGCGGCGCAAGCGTC
GCTACGCACTCGACAAGCTGGTTGCAGGTATAACGCCGGACAACCGTCATGAAGAGGTTGATTGGGGGCGACCCATTGGC
AACGAAGTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A170NWK0

Antitoxin

• AlphaFold DB: A0A166LMC5