Detailed information of TA system TA195314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2316301..2316818
• Replicon: chromosome
• Accession: NZ_CP071102
• Organism: Staphylococcus aureus strain CI/BAC/25/13/W

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: JYA70_RS11325
• Protein ID: WP_000113262.1
• Coordinates: 2316552..2316818 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: JYA70_RS11320
• Protein ID: WP_000584499.1
• Coordinates: 2316301..2316552 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA70_RS11290 (2311999)	2311999..2312718	+	720	WP_000479576.1	amino acid ABC transporter permease	-
JYA70_RS11295 (2312715)	2312715..2313446	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
JYA70_RS11300 (2313543)	2313543..2314793	-	1251	WP_211044206.1	FemA/FemB family glycyltransferase FmhA	-
JYA70_RS11305 (2315048)	2315048..2315410	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
JYA70_RS11310 (2315429)	2315429..2316028	+	600	WP_000162810.1	DsbA family protein	-
JYA70_RS11315 (2316056)	2316056..2316136	+	81	WP_223197974.1	hypothetical protein	-
JYA70_RS11320 (2316301)	2316301..2316552	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JYA70_RS11325 (2316552)	2316552..2316818	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
JYA70_RS13590 (2316853)	2316853..2316924	+	72	WP_223200558.1	hypothetical protein	-
JYA70_RS11330 (2317112)	2317112..2318662	+	1551	WP_000727764.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
JYA70_RS11335 (2318848)	2318848..2319315	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
JYA70_RS11340 (2319402)	2319402..2319605	+	204	WP_211044207.1	hypothetical protein	-
JYA70_RS11345 (2319800)	2319800..2320624	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
JYA70_RS11350 (2320858)	2320858..2321394	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T195314 WP_000113262.1 NZ_CP071102:2316552-2316818 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T195314 NZ_CP094523:4296630-4297031 [Bradyrhizobium sp. ISRA443]
ATGCCCGGTGACTTCTTCGACACCAACGTGCTTGTCCACCTCGCGTCGGGCGATCCCTTGAAGGCCGATCGGGCAGAAGC
CGTCATTTCCCGGGGCGGGGTGATTAGCGTTCAGGTGCTGAATGAAATCGCCAATGTCGCGCGCCGCAAGATGCGGATGT
CATGGACGGAGACGCGCGCATTTCTGACGATCTTGCGCAATCTGCTGACGATCTATCCCCTCACGCAGGAGATCCATGAC
GCTGGACTTGATCTGGCCGAGCGCTACGGATTGTCGATGTTCGACGCCATGATCGCCGCCTCCGCGCTTCAGGCCGGTTG
CGACAGATTGTGGTCTGAGGACATGCAGCACAACCAAGTCCTGGATAACAGCCTTCGGATCGTCAATCCGTTTCGGGTCT
AG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT195314 WP_000584499.1 NZ_CP071102:2316301-2316552 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT195314 NZ_CP094523:4296410-4296640 [Bradyrhizobium sp. ISRA443]
ATGCAAGTCGCCAAATGGGGTAACAGCCTCGCGATTCGGCTTCCCGCGGCCGTGGTCGAAGCGCTCGAACTCAAGGAAGG
CGACGAGATCGAAATCCATGTTGCCGACGAGCGGAACTTCGGCGTCGCGCGCAAACCGGGGCGTGAGGAGTTACTGGCGC
GACTGCGCGCCTTCCGCGGACGCCTGCCGGCGGATTTCAAATTCGACCGGGACGAGGCAAATGCCCGGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60