Detailed information of TA system TA195276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid p3347689I_1
• Accession: NZ_CP071087
• Organism: Klebsiella pneumoniae strain 3347689I

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JY394_RS27965
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JY394_RS27960
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY394_RS27910 (JY394_27910)	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
JY394_RS27915 (JY394_27915)	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
JY394_RS27920 (JY394_27920)	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
JY394_RS27925 (JY394_27925)	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
JY394_RS27930 (JY394_27930)	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
JY394_RS27935 (JY394_27935)	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
JY394_RS29490	12139..12330	+	192	WP_001270409.1	hypothetical protein	-
JY394_RS27945 (JY394_27945)	12333..12860	+	528	WP_004201083.1	thermonuclease family protein	-
JY394_RS27950 (JY394_27950)	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
JY394_RS27955 (JY394_27955)	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
JY394_RS27960 (JY394_27960)	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JY394_RS27965 (JY394_27965)	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JY394_RS27970 (JY394_27970)	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
JY394_RS27975 (JY394_27975)	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
JY394_RS27980 (JY394_27980)	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
JY394_RS27985 (JY394_27985)	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
JY394_RS27990 (JY394_27990)	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
JY394_RS27995 (JY394_27995)	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
JY394_RS28000 (JY394_28000)	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA14 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / armA / msr(E) / mph(E) / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / dfrA1 / tet(A) / catA1 / blaCTX-M-15	-	1..222789	222789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T195276 WP_000270043.1 NZ_CP071087:c14256-13906 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T195276 NZ_CP094479:c93801-93481 [Klebsiella pneumoniae]
ATGAAAACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGCAAATGTTACTGAC
AAGCCTGCTAGACCAGCGCTATGGTCTCACGCTAAACGACACGCCGTTCAGTGATGAAACTATTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCCGATGCCGTAAACTTTCTGGTTGAACGCTATGAACTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACCTCTATGGATGTTCACCGAGCACGATTTAAACTCAACGTGGAACGTTTATA
A

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT195276 WP_000124640.1 NZ_CP071087:c13901-13599 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT195276 NZ_CP094479:c94148-93822 [Klebsiella pneumoniae]
ATGTCATTAATACCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAGGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAATTTGATGGGCAATTTCAGCGATACGGAATGCTTCAAATTGAATGACG
CATTTCCACACTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAACTAATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGGTTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCGGTTTATCCCACTCA
ACGCTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9