Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332715..333301 Replicon chromosome
Accession NZ_CP071086
Organism Klebsiella pneumoniae strain 3347689I

Toxin (Protein)


Gene name doc Uniprot ID A0A483YV82
Locus tag JY394_RS01545 Protein ID WP_019725145.1
Coordinates 332933..333301 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A483YZZ9
Locus tag JY394_RS01540 Protein ID WP_019725146.1
Coordinates 332715..332936 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY394_RS01520 (JY394_01520) 328872..329798 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JY394_RS01525 (JY394_01525) 329795..331072 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
JY394_RS01530 (JY394_01530) 331069..331836 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JY394_RS01535 (JY394_01535) 331838..332551 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
JY394_RS01540 (JY394_01540) 332715..332936 + 222 WP_019725146.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JY394_RS01545 (JY394_01545) 332933..333301 + 369 WP_019725145.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JY394_RS01550 (JY394_01550) 333573..334889 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
JY394_RS01555 (JY394_01555) 334996..335883 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
JY394_RS01560 (JY394_01560) 335880..336725 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
JY394_RS01565 (JY394_01565) 336727..337797 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 329795..338534 8739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13542.89 Da        Isoelectric Point: 8.6410

>T195257 WP_019725145.1 NZ_CP071086:332933-333301 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTSGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFIKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T195257 NZ_CP094460:787982-788278 [Burkholderia pyrrocinia]
ATGGAAAAGAATTCGGCTCATTGCAAGCTGTCCCGGATCAAAACTCTCATCGAGAACGGCAAGGTGCGTTTGACCGCGAG
CGCGGTATTCGGCGCCCGGCAACTCGGTTTTACGGAACGGGAGGCAGTCGGTGTCGTCATGTCGCTGACAGCCGCCGATT
TCTACAAGAGCATGACGACGTATGCCGACCATACGATCTGGCAAGACGTCTATCAACGTCGCACAGTCCATGGCGACGTG
TACCTGAAACTGACAGTGATCGACGACGTGCTGATCGTGTCTTTCAAGGAGCGGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT195257 WP_019725146.1 NZ_CP071086:332715-332936 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDELRELADK

Download         Length: 222 bp

>AT195257 NZ_CP094460:788281-788682 [Burkholderia pyrrocinia]
ATGAAGTGCCCTGCCTGCGGTGCCGCCAAGCTGGTCCGCGATACACGAGATATCCCCTATACGTACAAGGGTCGATCGAC
GGTCGTCCGCGATATCACCGGTGATTTCTGCCCGGCCTGCGGAGAGTCGGTCCTCGACCTCGACGAGGCGACCCGTCTCG
GCGACGCGATCACCGAGTTCAACAAACAGGTGAACTCGACCATCGTCGACCCGAAGTACATTGCGAAGGTACGCAAGAAG
CTGATGCTGGACCAGCGCGAGGCGGCCGAGATATTCGGTGGCGGTGTCAACGCGTTCTCCCGCTACGAAACCGGCAAGAC
GAACCCGCCGCTCGCCCTTGTGAAACTGTTCAAGATACTGGATCGCCATCCGGATCTGCTTGCCGAAGTGCGGGCCGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YV82

Antitoxin

Source ID Structure
AlphaFold DB A0A483YZZ9

References