Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 13599..14256 Replicon plasmid p3347558_1
Accession NZ_CP071074
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag JY392_RS25105 Protein ID WP_000270043.1
Coordinates 13906..14256 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JY392_RS25100 Protein ID WP_000124640.1
Coordinates 13599..13901 (-) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS25050 (JY392_25045) 9233..9661 + 429 WP_000591076.1 hypothetical protein -
JY392_RS25055 (JY392_25050) 9719..10078 + 360 WP_000422769.1 hypothetical protein -
JY392_RS25060 (JY392_25055) 10078..10524 + 447 WP_000919343.1 hypothetical protein -
JY392_RS25065 (JY392_25060) 10521..11039 + 519 WP_000210757.1 nitrite reductase -
JY392_RS25070 (JY392_25065) 11039..11269 + 231 WP_000972665.1 hypothetical protein -
JY392_RS25075 (JY392_25070) 11256..12113 + 858 WP_001167036.1 hypothetical protein -
JY392_RS27990 12139..12330 + 192 WP_001270409.1 hypothetical protein -
JY392_RS25085 (JY392_25080) 12333..12860 + 528 WP_004201083.1 thermonuclease family protein -
JY392_RS25090 (JY392_25085) 12918..13190 + 273 WP_001043046.1 HU family DNA-binding protein -
JY392_RS25095 (JY392_25090) 13279..13572 + 294 WP_001239998.1 hypothetical protein -
JY392_RS25100 (JY392_25095) 13599..13901 - 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
JY392_RS25105 (JY392_25100) 13906..14256 - 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JY392_RS25110 (JY392_25105) 14419..14967 + 549 WP_001061573.1 hypothetical protein -
JY392_RS25115 (JY392_25110) 15308..15502 + 195 WP_000343597.1 hypothetical protein -
JY392_RS25120 (JY392_25115) 15513..15884 + 372 WP_000516918.1 hypothetical protein -
JY392_RS25125 (JY392_25120) 15877..16347 + 471 WP_001281821.1 hypothetical protein -
JY392_RS25130 (JY392_25125) 16362..16697 - 336 WP_000683477.1 hypothetical protein -
JY392_RS25135 (JY392_25130) 16794..17282 + 489 WP_001273096.1 DUF1643 domain-containing protein -
JY392_RS25140 (JY392_25135) 17285..17782 + 498 WP_000062185.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-4 / dfrA14 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / aph(3')-VI / blaNDM-1 / armA / msr(E) / mph(E) - 1..169082 169082


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T195230 WP_000270043.1 NZ_CP071074:c14256-13906 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T195230 NZ_CP094443:2455467-2455574 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT195230 WP_000124640.1 NZ_CP071074:c13901-13599 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT195230 NZ_CP094443:c2455651-2455591 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References