Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4297947..4298746 Replicon chromosome
Accession NZ_CP071073
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag JY392_RS20850 Protein ID WP_000347269.1
Coordinates 4298282..4298746 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag JY392_RS20845 Protein ID WP_001309780.1
Coordinates 4297947..4298282 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS20830 (4293732) 4293732..4294502 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JY392_RS20835 (4294518) 4294518..4295852 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JY392_RS20840 (4296227) 4296227..4297798 + 1572 WP_001273738.1 galactarate dehydratase -
JY392_RS20845 (4297947) 4297947..4298282 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JY392_RS20850 (4298282) 4298282..4298746 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JY392_RS20855 (4298801) 4298801..4299610 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JY392_RS20860 (4299859) 4299859..4301139 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
JY392_RS20865 (4301162) 4301162..4301635 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JY392_RS20870 (4301646) 4301646..4302425 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JY392_RS20875 (4302415) 4302415..4303293 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JY392_RS20880 (4303311) 4303311..4303745 + 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4288705..4298746 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T195228 WP_000347269.1 NZ_CP071073:4298282-4298746 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T195228 NZ_CP094443:1254858-1255046 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT195228 WP_001309780.1 NZ_CP071073:4297947-4298282 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT195228 NZ_CP094443:1254569-1254829 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References