Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2386477..2387039 Replicon chromosome
Accession NZ_CP071073
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JY392_RS11690 Protein ID WP_000605676.1
Coordinates 2386761..2387039 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JY392_RS11685 Protein ID WP_000781370.1
Coordinates 2386477..2386761 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS11670 (2381659) 2381659..2384706 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
JY392_RS11675 (2384719) 2384719..2385603 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
JY392_RS11680 (2385596) 2385596..2386249 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
JY392_RS11685 (2386477) 2386477..2386761 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JY392_RS11690 (2386761) 2386761..2387039 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JY392_RS11695 (2387225) 2387225..2388235 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JY392_RS11700 (2388369) 2388369..2390066 - 1698 WP_000433464.1 malate dehydrogenase -
JY392_RS11705 (2390223) 2390223..2390360 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JY392_RS11710 (2390462) 2390462..2390677 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JY392_RS11715 (2391022) 2391022..2391453 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T195215 WP_000605676.1 NZ_CP071073:c2387039-2386761 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T195215 NZ_CP094361:c1348956-1348210 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT195215 WP_000781370.1 NZ_CP071073:c2386761-2386477 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT195215 NZ_CP094361:c1348210-1348040 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References