Detailed information of TA system TA195215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2386477..2387039
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: higB
• Uniprot ID: L4J948
• Locus tag: JY392_RS11690
• Protein ID: WP_000605676.1
• Coordinates: 2386761..2387039 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1PAQ1
• Locus tag: JY392_RS11685
• Protein ID: WP_000781370.1
• Coordinates: 2386477..2386761 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS11670 (2381659)	2381659..2384706	+	3048	WP_012602782.1	formate dehydrogenase-N subunit alpha	-
JY392_RS11675 (2384719)	2384719..2385603	+	885	WP_001240565.1	formate dehydrogenase N subunit beta	-
JY392_RS11680 (2385596)	2385596..2386249	+	654	WP_000045646.1	formate dehydrogenase-N subunit gamma	-
JY392_RS11685 (2386477)	2386477..2386761	-	285	WP_000781370.1	HigA family addiction module antitoxin	Antitoxin
JY392_RS11690 (2386761)	2386761..2387039	-	279	WP_000605676.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JY392_RS11695 (2387225)	2387225..2388235	-	1011	WP_000642407.1	alcohol dehydrogenase AdhP	-
JY392_RS11700 (2388369)	2388369..2390066	-	1698	WP_000433464.1	malate dehydrogenase	-
JY392_RS11705 (2390223)	2390223..2390360	-	138	WP_000841554.1	stationary-phase-induced ribosome-associated protein	-
JY392_RS11710 (2390462)	2390462..2390677	-	216	WP_000495766.1	biofilm-dependent modulation protein	-
JY392_RS11715 (2391022)	2391022..2391453	+	432	WP_000152305.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-88)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10521.10 Da
• Isoelectric Point: 7.3562

>T195215 WP_000605676.1 NZ_CP071073:c2387039-2386761 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T195215 NZ_CP094361:c1348956-1348210 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10401.08 Da
• Isoelectric Point: 8.8567

>AT195215 WP_000781370.1 NZ_CP071073:c2386761-2386477 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Nucleotide

• Length: 285 bp

>AT195215 NZ_CP094361:c1348210-1348040 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829G6T1

Antitoxin

• AlphaFold DB: A0A829CUG6