Detailed information of TA system TA195206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 977523..978173
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JY392_RS04670
• Protein ID: WP_000263532.1
• Coordinates: 977523..977864 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: JY392_RS04675
• Protein ID: WP_000212552.1
• Coordinates: 977874..978173 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS04645 (972558)	972558..973658	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
JY392_RS04650 (973658)	973658..973957	+	300	WP_000867281.1	rod-binding protein	-
JY392_RS04655 (974133)	974133..975509	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
JY392_RS04660 (975524)	975524..976453	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
JY392_RS04665 (976470)	976470..977447	+	978	WP_001195938.1	hypothetical protein	-
JY392_RS04670 (977523)	977523..977864	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JY392_RS04675 (977874)	977874..978173	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
JY392_RS04680 (978231)	978231..979073	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
JY392_RS04685 (979559)	979559..980473	+	915	WP_000949083.1	lateral flagellin LafA	-
JY392_RS04690 (980678)	980678..981994	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
JY392_RS04695 (982017)	982017..982409	+	393	WP_000285322.1	flagellar export chaperone FliS	-
JY392_RS04700 (982414)	982414..982725	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T195206 WP_000263532.1 NZ_CP071073:977523-977864 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T195206 NZ_CP094359:c1390838-1390560 [Acidithiobacillus sp. YTS05]
ATGATTCGATCATTCCGCCACAAGGGGCTGGAGGCATTCTATGCATCGGGCTCTCTCGCCGGCATCCAAGCACAGCACGC
CAAGCGGCTCCGCATGCTACTTGCAGCCTTGGATACTGCACAGGCGATCGGCGACATGGACATCCCAGGATTTCGCCTGC
ATCCTCTCCAAGGTACGGAGAAGGGGCGCTGGTCGATTCGGGTCAACGCGAATTGGCGGTTGACCTTCGATTTTCAGGAC
GGTCATGCCTATGTCTTGGATTATGAGGACTACCACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT195206 WP_000212552.1 NZ_CP071073:977874-978173 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT195206 NZ_CP094359:c1390560-1390261 [Acidithiobacillus sp. YTS05]
ATGGAAATGCACAATCCCCCACATCCCGGCGAATTTATCCGTGAGGTCTACCTCGAACCGAATGGGATCACCGGACGGCA
GTTGGCGACCAAGCTTGGGGTATCGGCCTCTACCTTGGCCCGTGTCCTCAATGGTAGCAACGGCGTGAGCTCGGAGATGG
CCCTACGGTTATCCAAGGCGCTGGGGCGCTCTGCGGAAAGCTGGATGATCATGCAGGATCATTTTGACTTGTGGCAGGCA
CGACAGCGGGTAAACCTGGATCAGGTCCAGAGGCTGGATCTTCAGCCGGCAGACCAATGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7