Detailed information of TA system TA195112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4605231..4605833
• Replicon: chromosome
• Accession: NZ_CP071025
• Organism: Enterobacter hormaechei strain RIVM_C010068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: JQL77_RS21900
• Protein ID: WP_022649837.1
• Coordinates: 4605522..4605833 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQL77_RS21895
• Protein ID: WP_022649836.1
• Coordinates: 4605231..4605521 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQL77_RS21880	4602729..4603631	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
JQL77_RS21885	4603628..4604263	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
JQL77_RS21890	4604260..4605189	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
JQL77_RS21895	4605231..4605521	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
JQL77_RS21900	4605522..4605833	-	312	WP_022649837.1	hypothetical protein	Toxin
JQL77_RS21905	4605982..4606923	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
JQL77_RS21910	4606968..4607405	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JQL77_RS21915	4607402..4608283	-	882	WP_022649840.1	virulence factor BrkB family protein	-
JQL77_RS21920	4608277..4608876	-	600	WP_022649841.1	glucose-1-phosphatase	-
JQL77_RS21925	4608995..4609795	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
JQL77_RS21930	4609830..4610726	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4594663..4605833	11170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T195112 WP_022649837.1 NZ_CP071025:c4605833-4605522 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T195112 NZ_CP094322:c1116947-1116744 [Edwardsiella anguillarum]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTGGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAACTAGAAATTTTTTATTCTGCTGCAGATCATCGACTAGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCGGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT195112 WP_022649836.1 NZ_CP071025:c4605521-4605231 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT195112 NZ_CP094322:c1117336-1116968 [Edwardsiella anguillarum]
ATGGATGAGTATACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAACCTACAGCTTAACGAGCTGATTGAGC
ATATTGCCAGCGTCGCACAATCATTTAAGATCAAATATCCGCGTCATTCGGATTTAGCCGAAATACTGGATGATTATCTG
GATGAAACCTACGCCCTGTTTGGTGCCTATAGCGTCAGTGAAACGGCGCTCAGACACTGGTTGCGTACCAAACGCCGTGT
GGCATACTGCCTGGCGCATGAAAAGCGCAATGCGGCCTTACATGTTTAG

Structures

Toxin

• AlphaFold DB: A0A155DUZ6