Detailed information of TA system TA195032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4622434..4623036
• Replicon: chromosome
• Accession: NZ_CP071015
• Organism: Enterobacter hormaechei strain RIVM_C019181

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: JQL71_RS21995
• Protein ID: WP_022649837.1
• Coordinates: 4622725..4623036 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQL71_RS21990
• Protein ID: WP_022649836.1
• Coordinates: 4622434..4622724 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQL71_RS21975	4619932..4620834	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
JQL71_RS21980	4620831..4621466	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
JQL71_RS21985	4621463..4622392	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
JQL71_RS21990	4622434..4622724	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
JQL71_RS21995	4622725..4623036	-	312	WP_022649837.1	hypothetical protein	Toxin
JQL71_RS22000	4623185..4624126	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
JQL71_RS22005	4624171..4624608	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JQL71_RS22010	4624605..4625486	-	882	WP_022649840.1	virulence factor BrkB family protein	-
JQL71_RS22015	4625480..4626079	-	600	WP_022649841.1	glucose-1-phosphatase	-
JQL71_RS22020	4626198..4626998	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
JQL71_RS22025	4627033..4627929	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4611866..4623036	11170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T195032 WP_022649837.1 NZ_CP071015:c4623036-4622725 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T195032 NZ_CP094286:3367-3654 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT195032 WP_022649836.1 NZ_CP071015:c4622724-4622434 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT195032 NZ_CP094286:3641-3955 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6