Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 30480..31210 Replicon chromosome
Accession NZ_CP071015
Organism Enterobacter hormaechei strain RIVM_C019181

Toxin (Protein)


Gene name higB Uniprot ID A0A155SSD8
Locus tag JQL71_RS00155 Protein ID WP_022646552.1
Coordinates 30480..30794 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JQL71_RS00160 Protein ID WP_022646553.1
Coordinates 30791..31210 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQL71_RS00130 26146..27231 + 1086 Protein_25 cellulase family glycosylhydrolase -
JQL71_RS00135 27137..28321 - 1185 WP_022646548.1 multidrug efflux MFS transporter EmrD -
JQL71_RS00140 28497..29330 - 834 WP_022646549.1 DMT family transporter -
JQL71_RS00145 29393..29839 - 447 WP_022646550.1 GNAT family N-acetyltransferase -
JQL71_RS00150 29904..29993 - 90 WP_022646551.1 type I toxin-antitoxin system toxin TisB -
JQL71_RS00155 30480..30794 + 315 WP_022646552.1 type II toxin-antitoxin system HigB family toxin Toxin
JQL71_RS00160 30791..31210 + 420 WP_022646553.1 helix-turn-helix domain-containing protein Antitoxin
JQL71_RS00165 31357..31455 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
JQL71_RS00170 31685..33373 + 1689 WP_022646554.1 acetolactate synthase large subunit -
JQL71_RS00175 33377..33664 + 288 WP_015570113.1 acetolactate synthase small subunit -
JQL71_RS00180 33790..34383 + 594 WP_022646555.1 transcriptional regulator UhpA -
JQL71_RS00185 34380..35885 + 1506 WP_022646556.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Antitoxin HigA

(1-139)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12441.55 Da        Isoelectric Point: 10.1989

>T195018 WP_022646552.1 NZ_CP071015:30480-30794 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T195018 NZ_CP094276:198943-199227 [Klebsiella aerogenes]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15480.10 Da        Isoelectric Point: 5.6761

>AT195018 WP_022646553.1 NZ_CP071015:30791-31210 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT195018 NZ_CP094276:198705-198953 [Klebsiella aerogenes]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155SSD8

Antitoxin

Source ID Structure

References