Detailed information of TA system TA194966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1734869..1735383
• Replicon: chromosome
• Accession: NZ_CP071000
• Organism: Limosilactobacillus fermentum strain GR1008

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6C1EMT8
• Locus tag: JYQ65_RS08875
• Protein ID: WP_035437199.1
• Coordinates: 1734869..1735132 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: JYQ65_RS08880
• Protein ID: WP_035437201.1
• Coordinates: 1735129..1735383 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYQ65_RS08860	1730345..1731127	+	783	WP_035437193.1	dimethylargininase	-
JYQ65_RS08865	1731515..1732912	+	1398	WP_035437196.1	sodium:proton antiporter	-
JYQ65_RS08870	1733338..1734459	+	1122	WP_080650628.1	PTS sugar transporter subunit IIC	-
JYQ65_RS08875	1734869..1735132	-	264	WP_035437199.1	Txe/YoeB family addiction module toxin	Toxin
JYQ65_RS08880	1735129..1735383	-	255	WP_035437201.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JYQ65_RS08885	1735632..1735919	+	288	WP_041807836.1	transposase	-
JYQ65_RS08890	1735943..1736821	+	879	WP_086031809.1	IS3 family transposase	-
JYQ65_RS08895	1737619..1738383	+	765	WP_080650602.1	zinc ribbon domain-containing protein	-
JYQ65_RS08900	1738590..1739495	-	906	WP_154244344.1	IS3 family transposase	-
JYQ65_RS08905	1739432..1740046	-	615	WP_035431426.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-78)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10252.94 Da
• Isoelectric Point: 10.3349

>T194966 WP_035437199.1 NZ_CP071000:c1735132-1734869 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVLPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHDE

Nucleotide

• Length: 264 bp

>T194966 NZ_CP094240:2236245-2236541 [Comamonas sp. 7D-2evo2]
ATGACCAAGATTGCAGACCTGCCCACCTTTGATATGGCAGAGCACCTGAAAACCGATGAAGACCTGGCTGCCTACCTATC
CTTGGTGCTGGAAGAAGACGACCCCGCAGAGCTGGCCAACGCTCTGGGTGTGATTGCCCGCGCCCGTGGCATGACCGAGA
TTGCCAGCGCCAGCGGCCTGACCCGCGAGGCTCTTTACAAGGCGCTACGCCCTGGCAGCCAGCCGCGCTTTGACACCATC
CGCCGGGTCTGTGGTGCCCTTGGGGTTCGCTTGGTGGCCCAGCCTATCCACGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9773.05 Da
• Isoelectric Point: 4.4237

>AT194966 WP_035437201.1 NZ_CP071000:c1735383-1735129 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTRADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT194966 NZ_CP094240:2235946-2236242 [Comamonas sp. 7D-2evo2]
ATGTTCACCATCAAGCGAACCGCTGAATTTGATGCATGGCTATTCGGCCTGCGCGACACCATGACGCGCATCCGCCTTGC
CAAGCGACTGGACAAGGTGCAGCGTGGCCCGCTTGGAGATACAAAGCCCGTGGGTGATGGCGTGCATGAGCTGCGCGAGG
ACTTCGGCCCTGGCTGGTGCATGTACTACGTGCAGCACGGTGATGTGCTGGTGGTGATGCTGGGCGGAGGGGACAAGTCC
ACCCAGCAGGCCGACATTCAGGCCGCGATTGCTTTGTCCAAGACCCTGGAGGACTGA

Structures

Toxin

• AlphaFold DB: A0A6C1EMT8