Detailed information of TA system TA194872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 44807..45464
• Replicon: plasmid p706-1
• Accession: NZ_CP070971
• Organism: Klebsiella pneumoniae strain JS706

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JXZ99_RS26500
• Protein ID: WP_000270043.1
• Coordinates: 45114..45464 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JXZ99_RS26495
• Protein ID: WP_000124640.1
• Coordinates: 44807..45109 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JXZ99_RS26450	40432..40860	+	429	WP_000591074.1	hypothetical protein	-
JXZ99_RS26455	40917..41276	+	360	WP_000422768.1	hypothetical protein	-
JXZ99_RS26460	41276..41722	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
JXZ99_RS26465	41719..42237	+	519	WP_000210756.1	nitrite reductase	-
JXZ99_RS26470	42237..42467	+	231	WP_000972663.1	hypothetical protein	-
JXZ99_RS26475	42454..43311	+	858	WP_001167032.1	hypothetical protein	-
JXZ99_RS26480	43542..44069	+	528	WP_001236377.1	thermonuclease family protein	-
JXZ99_RS26485	44127..44399	+	273	WP_001043047.1	HU family DNA-binding protein	-
JXZ99_RS26490	44487..44780	+	294	WP_001239997.1	chromosome segregation protein ParM	-
JXZ99_RS26495	44807..45109	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JXZ99_RS26500	45114..45464	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JXZ99_RS26505	45627..46175	+	549	WP_001061574.1	transcriptional regulator	-
JXZ99_RS26510	46516..46710	+	195	WP_000343597.1	hypothetical protein	-
JXZ99_RS26515	46721..47092	+	372	WP_000516916.1	hypothetical protein	-
JXZ99_RS26520	47085..47555	+	471	WP_001281821.1	hypothetical protein	-
JXZ99_RS26525	47570..47905	-	336	WP_000683476.1	hypothetical protein	-
JXZ99_RS26530	48002..48490	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
JXZ99_RS26535	48493..48990	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-14 / blaTEM-1B / rmtB / tet(G) / floR / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2	-	1..106354	106354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T194872 WP_000270043.1 NZ_CP070971:c45464-45114 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T194872 NZ_CP094143:20722-21486 [Helicobacter pylori]
ATGGAAACTAAAGAAGAAGATAAAGATAAAAAGCTAGAAAAAATTATTGTATTGCTGTGCGAAGAGGGGGATTTGTCCAG
CCAAAAAGATCAAATCATCAAAGATCTCAAAGAAATCTATAAAGGAGAATACAAGCATAAATATTCAAAAATCACAACTA
TTATTTTAAATTCCACAAGGGATAAAGAGCAAGCCTTTATGATGCTCACACAAAATATAAAGACACTCAAAGAGATTCAA
GATAATAAAGAAGTTGAAAGCATTAAACCAAAACTAGAAAAGCTTTATGATCACATGAATTTAGAGTGTATCAGACTGCA
AGATTTTGATGAGAAAATGAGTAGAGTTAAAAATGTTTCTATAAAGTTAGAAGATGAACTAAATAAAAACTATAAAAAAT
TAAGCGAAGAATTGAATAAGCAACAAACACAATACATAACAATTCTAGGTATTTTTGCCTCTATTGTTTTAACATTTGTT
GGGGGATTGGCGTTTTCCACTTCTGTTTTATCAAATATTGACAAAGCGAACGCCTATCGTTTGGTTTTTGTTATGGCTTT
TATAGCTTTATTTTTTGGGAATATTTTGTATCTGCTTTTTTCTTTTTTATCAAAAATATCCTTATCAGAAGAGAAAAAAG
ATAAACAAGAAAATTTTTTCAAAAAACCTATTTTTTGGTTTAATCTAATGGTTATAATTCTATTTGTGATTGGTTTTTTT
GGAGAATTGCATATAATACAAAGATTAGTTTCTAAATATCTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT194872 WP_000124640.1 NZ_CP070971:c45109-44807 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT194872 NZ_CP094143:20295-20732 [Helicobacter pylori]
ATGGTCAATACTTTGGAATTAGCAAAATATATTCTTAAGCATTCAAACAAAGAATTGAGCAACTTAGAATTGCAAAAAAC
TTTGTATTTCACAGAGCTTGACTATATTAAAAAGTTTGACAAACACCTAGTTTCTGATGATTTTGAAGCTTGGAAATACG
GGCCTGTTGCAAGAGAAGTGTATTATGAATATCGCAATTACGGTGCCAATTCTATTGACAAACCTAAAGAAGAAACGCTC
TCACAAAATCTTAGAGAAGACGAACTTGAAACCATTAATTGCTCCATAAAAAAATGTAACGAGAAATCCTATTGGGATTT
AGTGAAAGAGAGTCATAAAGAAGATGGCGCTTGGCATAAAAGCTTCAGAGAAGATAGAAAAGAAATCATTAGCAAAGATT
TGATCAAACAAGAAGCAAAACAAGCAAATGGAAACTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9