Detailed information of TA system TA194866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3747557..3748203
• Replicon: chromosome
• Accession: NZ_CP070970
• Organism: Klebsiella pneumoniae strain JS706

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W8UNE1
• Locus tag: JXZ99_RS18700
• Protein ID: WP_002920560.1
• Coordinates: 3747856..3748203 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: JXZ99_RS18695
• Protein ID: WP_002920557.1
• Coordinates: 3747557..3747856 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JXZ99_RS18675	3743763..3744521	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
JXZ99_RS18680	3744571..3745401	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
JXZ99_RS18685	3745452..3745781	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
JXZ99_RS18690	3745986..3747494	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
JXZ99_RS18695	3747557..3747856	-	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
JXZ99_RS18700	3747856..3748203	-	348	WP_002920560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JXZ99_RS18705	3748379..3750826	-	2448	WP_002920561.1	glycogen phosphorylase	-
JXZ99_RS18710	3750844..3752277	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13502.56 Da
• Isoelectric Point: 6.2300

>T194866 WP_002920560.1 NZ_CP070970:c3748203-3747856 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T194866 NZ_CP094139:18276-19058 [Helicobacter pylori]
ATGGAAATTAAAGAAGAGGATAAAGATAAAAAGCTAGAAAAAATTATTGTATTGCTGTGCGAAGAGGGGGATTTGTCTAG
CCAAAAAGATCAAATCATCAAAGATCTCAAAGAAATCTATAAAGGAGAATACAAGCATAAATACTCAAAAATCACAACTA
TTATTTTAAATTCCACAAGGGATAAAGAGCAAGCCTTTATGATGCTCACACAAAATATAAAGACACTCAAAGAGATTCAA
GATAATCAAGAAATTGAAAGCATTAAGCCAAAACTAGAAAAGCTTTATGATCACATGAATTTAGAGTGTATTAGACTGCA
AGATTTTGATGAGAAAATGAGTAGAGTTAAAGATGTTTCTATCAGGTTAGAAGATGATCTAAATAAAAACTATAAAAAAT
TAAGCGAAGAATTGAATAAGCAGCAAACCCAATATATAACCATTTTAGGTATTTTTGCCTCTATTGTTTTAACATTTGTT
GGGGGATTGGCGTTTTCCACTTCTGTTTTATCAAATATTGACAAAGCGAACGCCTATCGTTTGGTTTTTGTTATGGCTTT
TATAGCTTTATTTTTTGGGAATATTTTGTATCTGCTTTTTTCTTTTTTATCAAAAATATCCTTATCAGAAGAGAAAAAAG
ATAAACAAGAAAATTTTTTCAAAAAACCTATTTTTTGGTTTAATCTAATGGTTACAATTCTATTTGTGATTGGTTTTGTG
GGAGAGAGAATTGATAGGCAATATAAAGCTCAGATTTCTAAAGATTTTAAAAGACCAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT194866 WP_002920557.1 NZ_CP070970:c3747856-3747557 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT194866 NZ_CP094139:17849-18286 [Helicobacter pylori]
ATGGTCAATGCTTTGGAACTAGCAAAATATATTCTTAAGCATTCAAACAAAGAATTGAGCAACTTAGAATTGCAAAAAAC
TTTGTATTTCACAGAGCTTGACTATATTAAAAAGTTTGACAAACACCTAGTTTCTGATGATTTTGAAGCTTGGAAATACG
GACCTGTTGCAAGGGAAGTGTATTATGAATATCGCAATTATGGTGCCAATTCTATTGACAAACCCAAAGAAGAAACGCTC
TCACAAAATCTTAGAGAAGATGAACTTGAAACCATTAATTGCTCCATAAAAAAATGCAACGAAAAATCCTATTGGGATTT
AGTGAAAGAGAGTCATAAAGAAGATGGTGCTTGGCATAAAAGCTTCAAAGAAGATAGAAAAGAAATCATTAGCAAAGATT
TGATCAAACAAGAAGCAAAACAAGCAAATGGAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0E1CBY5

Antitoxin

• AlphaFold DB: A0A0E1CBF8