Detailed information of TA system TA194833

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66192..66618
• Replicon: plasmid pMG101_B
• Accession: NZ_CP070963
• Organism: Escherichia coli strain K-12 J53

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JTR57_RS22785
• Protein ID: WP_001372321.1
• Coordinates: 66192..66317 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66394..66618 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTR57_RS22750 (61305)	61305..61532	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
JTR57_RS22755 (61620)	61620..62297	-	678	WP_001348626.1	PAS domain-containing protein	-
JTR57_RS22760 (62431)	62431..62814	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JTR57_RS22765 (63238)	63238..63747	+	510	WP_000759163.1	transglycosylase SLT domain-containing protein	-
JTR57_RS22770 (64043)	64043..64864	-	822	WP_001234468.1	DUF932 domain-containing protein	-
JTR57_RS22775 (64983)	64983..65270	-	288	WP_000107520.1	hypothetical protein	-
JTR57_RS23430 (65571)	65571..65744	+	174	Protein_72	hypothetical protein	-
JTR57_RS22780 (65742)	65742..65972	-	231	WP_074158030.1	hypothetical protein	-
JTR57_RS22785 (66192)	66192..66317	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JTR57_RS23435 (66259)	66259..66408	-	150	Protein_75	plasmid maintenance protein Mok	-
- (66394)	66394..66618	-	225	NuclAT_0	-	Antitoxin
- (66394)	66394..66618	-	225	NuclAT_0	-	Antitoxin
- (66394)	66394..66618	-	225	NuclAT_0	-	Antitoxin
- (66394)	66394..66618	-	225	NuclAT_0	-	Antitoxin
JTR57_RS22790 (66430)	66430..66618	+	189	WP_001299721.1	hypothetical protein	-
JTR57_RS22795 (66587)	66587..67349	-	763	Protein_77	plasmid SOS inhibition protein A	-
JTR57_RS22800 (67346)	67346..67780	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
JTR57_RS22805 (67835)	67835..69793	-	1959	WP_000117212.1	ParB/RepB/Spo0J family partition protein	-
JTR57_RS22810 (69858)	69858..70091	-	234	WP_000006030.1	DUF905 family protein	-
JTR57_RS22815 (70153)	70153..70692	-	540	WP_000290812.1	single-stranded DNA-binding protein	-
JTR57_RS22820 (70718)	70718..70924	-	207	WP_000547971.1	hypothetical protein	-
JTR57_RS22825 (71165)	71165..71434	-	270	WP_071977877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / blaOXA-1 / sitABCD / tet(B) / catA1	iutA / iucD / iucC / iucB / iucA	1..154497	154497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T194833 WP_001372321.1 NZ_CP070963:c66317-66192 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T194833 NZ_CP094109:579193-579453 [Helicobacter pylori]
ATGCTGAAAATTAAGCACCATATCCTTTACAAAAAAGATTTTAAAAAACTTATTAAAAACGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAAGAAAGAAAGAACCATTATCCCCACAATTTCAAGATCATGCCTTAAAGGGAAAGTGGAAACCTT
TTAGGGAATGCCACATCAAAGCTGATATTTTACTTGTGTATTTAGTGAAAGGTGATGAGCTTATTTTAGTAAGGCTAGGC
AGTCATAGCGAGCTGTTTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT194833 NZ_CP070963:c66618-66394 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin