Detailed information of TA system TA194829

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26585..26824
• Replicon: plasmid pMG101_B
• Accession: NZ_CP070963
• Organism: Escherichia coli strain K-12 J53

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: JTR57_RS22565
• Protein ID: WP_023144756.1
• Coordinates: 26585..26719 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	26764..26824 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTR57_RS22535 (22287)	22287..23144	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
JTR57_RS22540 (23137)	23137..23211	-	75	WP_031943482.1	RepA leader peptide Tap	-
JTR57_RS22550 (23448)	23448..23702	-	255	WP_000083850.1	replication regulatory protein RepA	-
JTR57_RS22555 (24075)	24075..25616	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
JTR57_RS22560 (25631)	25631..26377	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
JTR57_RS22565 (26585)	26585..26719	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (26764)	26764..26824	+	61	NuclAT_1	-	Antitoxin
- (26764)	26764..26824	+	61	NuclAT_1	-	Antitoxin
- (26764)	26764..26824	+	61	NuclAT_1	-	Antitoxin
- (26764)	26764..26824	+	61	NuclAT_1	-	Antitoxin
JTR57_RS22570 (26791)	26791..27077	-	287	Protein_30	DUF2726 domain-containing protein	-
JTR57_RS22575 (27590)	27590..27802	-	213	WP_013023861.1	hypothetical protein	-
JTR57_RS22580 (27933)	27933..28493	-	561	WP_000139341.1	fertility inhibition protein FinO	-
JTR57_RS22585 (28548)	28548..29294	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / blaOXA-1 / sitABCD / tet(B) / catA1	iutA / iucD / iucC / iucB / iucA	1..154497	154497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T194829 WP_023144756.1 NZ_CP070963:c26719-26585 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T194829 NZ_CP094105:625063-625329 [Helicobacter pylori]
ATGCTAAAAATTAAGCACCATATCCTTTACAAAAAAGATTTTAAAAAACTTATTAAAAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGACCCACAATTTAAAGATCATGTCTTGAAAGGAAAGTGGA
AGCCTTTTAGGGAATGCCACATCAAAGCTGATATTTTGCTTGTGTATTTAGTGAAAGGTGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT194829 NZ_CP070963:26764-26824 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin