Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3292167..3292708 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JTR57_RS16170 Protein ID WP_000615983.1
Coordinates 3292430..3292708 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JTR57_RS16165 Protein ID WP_000729703.1
Coordinates 3292167..3292427 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS16145 (3287842) 3287842..3288627 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JTR57_RS16150 (3288599) 3288599..3290311 + 1713 Protein_3176 flagellar biosynthesis protein FlhA -
JTR57_RS16155 (3290535) 3290535..3291032 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JTR57_RS16160 (3291208) 3291208..3291957 - 750 WP_000056849.1 C40 family peptidase -
JTR57_RS16165 (3292167) 3292167..3292427 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JTR57_RS16170 (3292430) 3292430..3292708 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JTR57_RS16175 (3292864) 3292864..3293604 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JTR57_RS16180 (3293575) 3293575..3294342 - 768 WP_000333380.1 class II glutamine amidotransferase -
JTR57_RS16185 (3294548) 3294548..3295126 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3271256..3317055 45799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T194821 WP_000615983.1 NZ_CP070962:3292430-3292708 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T194821 NZ_CP094096:574056-574328 [Helicobacter pylori]
ATGCTGATGATTGAAACTAGTAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
ATATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGATCCCAAATACAAAGACCACCCGCTCAAAGGCGCTTTAA
AAGATTTTAGGGAATGCCACCTAAAACCGGATTTATTGCTTGTCTATCAAATTAAAAAACAAGAAAACACCCTCTTTTTA
GTAAGGCTAGGCAGTCATAACGAGCTGTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT194821 WP_000729703.1 NZ_CP070962:3292167-3292427 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT194821 NZ_CP094096:573758-574069 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACCCAATACAGCGAAAAACAGCTTTTTAGCTTTTTAAATTCTATCAAGAC
CAAACAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGTTCAAAAACAAAGAATTAAAAAAGCGCTCCAATTTAAAG
CACTCCATTTAACTGAAAACGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGACACCAAGAACCAC
CTTTGTTTTAAAAGCGTAGAGGATTTTAAAAAGCATTGTGAAAACTTAAGATTTAAAAATGCTGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References