Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3285970..3286664 | Replicon | chromosome |
| Accession | NZ_CP070962 | ||
| Organism | Escherichia coli strain K-12 J53 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | JTR57_RS16130 | Protein ID | WP_001263489.1 |
| Coordinates | 3285970..3286368 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | JTR57_RS16135 | Protein ID | WP_000554758.1 |
| Coordinates | 3286371..3286664 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3281558) | 3281558..3281638 | - | 81 | NuclAT_12 | - | - |
| - (3281558) | 3281558..3281638 | - | 81 | NuclAT_12 | - | - |
| - (3281558) | 3281558..3281638 | - | 81 | NuclAT_12 | - | - |
| - (3281558) | 3281558..3281638 | - | 81 | NuclAT_12 | - | - |
| JTR57_RS16105 (3282234) | 3282234..3282692 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JTR57_RS16110 (3282953) | 3282953..3284410 | + | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| JTR57_RS16115 (3284467) | 3284467..3284988 | - | 522 | Protein_3169 | peptide chain release factor H | - |
| JTR57_RS16120 (3284984) | 3284984..3285190 | - | 207 | Protein_3170 | RtcB family protein | - |
| JTR57_RS16125 (3285508) | 3285508..3285960 | - | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| JTR57_RS16130 (3285970) | 3285970..3286368 | - | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JTR57_RS16135 (3286371) | 3286371..3286664 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JTR57_RS16140 (3286716) | 3286716..3287771 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| JTR57_RS16145 (3287842) | 3287842..3288627 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| JTR57_RS16150 (3288599) | 3288599..3290311 | + | 1713 | Protein_3176 | flagellar biosynthesis protein FlhA | - |
| JTR57_RS16155 (3290535) | 3290535..3291032 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | gmhA/lpcA | 3271256..3317055 | 45799 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T194820 WP_001263489.1 NZ_CP070962:c3286368-3285970 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T194820 NZ_CP094094:c1069851-1069567 [Helicobacter pylori]
ATGTATGCTTTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTACAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAATGGACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTC
TAGCACAAGTCTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG
ATGTATGCTTTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTACAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAATGGACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTC
TAGCACAAGTCTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT194820 WP_000554758.1 NZ_CP070962:c3286664-3286371 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT194820 NZ_CP094094:c1070224-1069832 [Helicobacter pylori]
ATGCCTAACTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCG
AAAAATCAAAAAGATTCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGTCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGATGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGGGTTAATCTCACTCAAATTGATAG
TTTAGATGATCTTACAAACGAGTTAAAAAGAGAAAATGGTAAAGGTTTTGAAAATGTATGCTTTAGCGTTTGA
ATGCCTAACTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCG
AAAAATCAAAAAGATTCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGTCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGATGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGGGTTAATCTCACTCAAATTGATAG
TTTAGATGATCTTACAAACGAGTTAAAAAGAGAAAATGGTAAAGGTTTTGAAAATGTATGCTTTAGCGTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |