Detailed information of TA system TA194801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 406518..407211
• Replicon: chromosome
• Accession: NZ_CP070962
• Organism: Escherichia coli strain K-12 J53

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: JTR57_RS02060
• Protein ID: WP_000415584.1
• Coordinates: 406518..406814 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JTR57_RS02065
• Protein ID: WP_000650107.1
• Coordinates: 406816..407211 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTR57_RS02025 (401606)	401606..401920	-	315	WP_000958598.1	putative quinol monooxygenase	-
JTR57_RS02030 (401951)	401951..402532	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JTR57_RS02035 (402851)	402851..403183	+	333	WP_000917684.1	DUF2645 family protein	-
JTR57_RS02040 (403229)	403229..404578	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
JTR57_RS02045 (404575)	404575..405234	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
JTR57_RS02050 (405386)	405386..405778	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JTR57_RS02055 (405831)	405831..406313	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
JTR57_RS02060 (406518)	406518..406814	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
JTR57_RS02065 (406816)	406816..407211	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JTR57_RS02070 (407344)	407344..408951	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
JTR57_RS02075 (409089)	409089..411347	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T194801 WP_000415584.1 NZ_CP070962:406518-406814 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T194801 NZ_CP094080:c150631-150350 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT194801 WP_000650107.1 NZ_CP070962:406816-407211 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT194801 NZ_CP094080:c150938-150624 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATG
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65