Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 340607..341334 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JTR57_RS01750 Protein ID WP_000550189.1
Coordinates 340607..340921 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JTR57_RS01755 Protein ID WP_000560266.1
Coordinates 340918..341334 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS01730 (336765) 336765..337751 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JTR57_RS01735 (337830) 337830..338522 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JTR57_RS01740 (338599) 338599..339102 - 504 WP_001333820.1 M48 family metallopeptidase -
JTR57_RS01745 (339187) 339187..340323 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JTR57_RS01750 (340607) 340607..340921 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JTR57_RS01755 (340918) 340918..341334 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JTR57_RS01760 (341379) 341379..343397 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JTR57_RS01765 (343823) 343823..346174 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T194800 WP_000550189.1 NZ_CP070962:340607-340921 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T194800 NZ_CP094078:575405-575671 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGACCCACAATTTAAAGATCATGCTTTAAAAGGCAATTGGA
AGCCTTTTAGGGAATGCCACATCAAAGCTGATATTTTGCTTGTTTATTTAGTGAAAGGTGATGAGCTTATTTTAGTAAGG
TTAGGTAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT194800 WP_000560266.1 NZ_CP070962:340918-341334 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT194800 NZ_CP094078:575047-575424 [Helicobacter pylori]
ATGCCTAATTCTCCCGCCAAAAAAGACTACACACGATACAGCGAAAAACAGCTTTTTAACTTAATCAATCAATTAGAGCA
AAAGATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAAGATAAAATAGACGCATTAAATAAACTCTTGCAAAAAATCAGCCAAGCTTTTGACGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCTATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAACCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References