Detailed information of TA system TA194793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1947969..1948625
• Replicon: chromosome
• Accession: NZ_CP070959
• Organism: Thiomicrospira microaerophila strain BDL05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JX580_RS09405
• Protein ID: WP_248850290.1
• Coordinates: 1948281..1948625 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JX580_RS09400
• Protein ID: WP_283103583.1
• Coordinates: 1947969..1948271 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JX580_RS09385 (JX580_09400)	1944282..1946633	-	2352	WP_248850287.1	ATP-binding protein	-
JX580_RS09390 (JX580_09405)	1946887..1947402	-	516	WP_248850288.1	winged helix-turn-helix domain-containing protein	-
JX580_RS09395 (JX580_09410)	1947514..1947789	-	276	WP_248850289.1	helix-turn-helix transcriptional regulator	-
JX580_RS09400 (JX580_09415)	1947969..1948271	-	303	WP_283103583.1	XRE family transcriptional regulator	Antitoxin
JX580_RS09405 (JX580_09420)	1948281..1948625	-	345	WP_248850290.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JX580_RS09410 (JX580_09425)	1948685..1948870	-	186	WP_283103617.1	hypothetical protein	-
JX580_RS09415 (JX580_09430)	1949215..1950132	-	918	WP_248850292.1	hypothetical protein	-
JX580_RS09420 (JX580_09435)	1950476..1951108	-	633	WP_248850293.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (29-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13162.30 Da
• Isoelectric Point: 8.9348

>T194793 WP_248850290.1 NZ_CP070959:c1948625-1948281 [Thiomicrospira microaerophila]
MWQVVTVDYFDDWFLSLNAAEQQGILTAIFKLQSIGPLLGRPDVDTLSGVHRVKNLKELRVQHKGKPYRVLFAFDPKRQA
VMLCGGDKTGDKRFYDRMIPIAEREFIAHLEKLD

Nucleotide

• Length: 345 bp

>T194793 NZ_CP094070:c1430313-1430032 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAAAGCGA
TGTTGTAAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCGCTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAGCCAAACTTGCTGTTGATTTATGAAGTCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11033.79 Da
• Isoelectric Point: 6.2258

>AT194793 WP_283103583.1 NZ_CP070959:c1948271-1947969 [Thiomicrospira microaerophila]
MAKPLADLLLKIDPAIVEAAQNQAEEDVLAWRLAYLREQLALSQVEMAERLGISQPSVANLEKRGHEVKLSSLKRYVEAL
GGKLSLDVQLPDGRHIGIHV

Nucleotide

• Length: 303 bp

>AT194793 NZ_CP094070:c1430620-1430306 [Helicobacter pylori]
ATGTCAAATATTACTACAGATTACTCACAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA