Detailed information of TA system TA194771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3319855..3320534
• Replicon: chromosome
• Accession: NZ_CP070954
• Organism: Escherichia fergusonii strain EF44

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: JYG65_RS15695
• Protein ID: WP_002431667.1
• Coordinates: 3320232..3320534 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JYG65_RS15690
• Protein ID: WP_000806441.1
• Coordinates: 3319855..3320196 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYG65_RS15680 (3315641)	3315641..3316930	-	1290	WP_000102764.1	APC family permease	-
JYG65_RS15685 (3317237)	3317237..3319741	+	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
JYG65_RS15690 (3319855)	3319855..3320196	-	342	WP_000806441.1	HigA family addiction module antitoxin	Antitoxin
JYG65_RS15695 (3320232)	3320232..3320534	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JYG65_RS15700 (3320667)	3320667..3321461	+	795	WP_148048123.1	TraB/GumN family protein	-
JYG65_RS15705 (3321665)	3321665..3322144	+	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
JYG65_RS15710 (3322168)	3322168..3322968	+	801	WP_236942037.1	hypothetical protein	-
JYG65_RS15715 (3322965)	3322965..3323468	+	504	WP_223666686.1	hypothetical protein	-
JYG65_RS15720 (3323506)	3323506..3325158	-	1653	WP_181591242.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T194771 WP_002431667.1 NZ_CP070954:c3320534-3320232 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T194771 NZ_CP094052:c134716-134435 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAGCGA
TGTTACAAGCGTTGTTGATCGTCTCAAAGAGCAAAAACCGCTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT194771 WP_000806441.1 NZ_CP070954:c3320196-3319855 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT194771 NZ_CP094052:c135023-134709 [Helicobacter pylori]
GTGTCAAATATTATTACAGATTACTCACAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GTTTAAGGCAGAAGGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTGAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8