Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2170461..2171023 Replicon chromosome
Accession NZ_CP070951
Organism Escherichia fergusonii strain EF31

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag JW961_RS10335 Protein ID WP_104919749.1
Coordinates 2170745..2171023 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JW961_RS10330 Protein ID WP_000781370.1
Coordinates 2170461..2170745 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW961_RS10315 (2165820) 2165820..2168867 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
JW961_RS10320 (2168880) 2168880..2169764 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
JW961_RS10325 (2169757) 2169757..2170410 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
JW961_RS10330 (2170461) 2170461..2170745 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JW961_RS10335 (2170745) 2170745..2171023 - 279 WP_104919749.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW961_RS10340 (2171209) 2171209..2172219 - 1011 WP_223662520.1 alcohol dehydrogenase AdhP -
JW961_RS10345 (2172354) 2172354..2174051 - 1698 WP_000433464.1 malate dehydrogenase -
JW961_RS10350 (2174208) 2174208..2174345 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JW961_RS10355 (2174447) 2174447..2174662 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JW961_RS10360 (2175004) 2175004..2175435 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T194740 WP_104919749.1 NZ_CP070951:c2171023-2170745 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADLDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T194740 NZ_CP093954:c180041-179784 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT194740 WP_000781370.1 NZ_CP070951:c2170745-2170461 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT194740 NZ_CP093954:c180303-180034 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References