Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 553851..554650 Replicon chromosome
Accession NZ_CP070951
Organism Escherichia fergusonii strain EF31

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JW961_RS02640 Protein ID WP_000347267.1
Coordinates 553851..554315 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID B7LMV4
Locus tag JW961_RS02645 Protein ID WP_015953962.1
Coordinates 554315..554650 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW961_RS02610 (548852) 548852..549286 - 435 WP_032303830.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JW961_RS02615 (549304) 549304..550182 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JW961_RS02620 (550172) 550172..550951 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JW961_RS02625 (550962) 550962..551435 - 474 WP_223662900.1 PTS N-acetylgalactosamine transporter subunit IIB -
JW961_RS02630 (551458) 551458..552738 - 1281 WP_223662898.1 tagatose-bisphosphate aldolase subunit KbaZ -
JW961_RS02635 (552987) 552987..553796 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JW961_RS02640 (553851) 553851..554315 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JW961_RS02645 (554315) 554315..554650 - 336 WP_015953962.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JW961_RS02650 (554799) 554799..556370 - 1572 WP_223662896.1 galactarate dehydratase -
JW961_RS02655 (556841) 556841..557611 + 771 WP_001058567.1 2-dehydro-3-deoxyglucarate aldolase -
JW961_RS02660 (557636) 557636..558526 + 891 WP_223662894.1 2-hydroxy-3-oxopropionate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T194726 WP_000347267.1 NZ_CP070951:c554315-553851 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T194726 NZ_CP093940:c123684-123502 [Pseudomonas sp. I3-I5]
GTGCACAGCCGAGACATCATCAAGCGGCTGAAGGATGACGGTTGGGTCAGAGTCAAAGGGACTGATGGGAGCCACCAGCA
GTTCAAGCATCCAACCAAGAAGGGGCGGGTTACGGTTCCTCATCCAAATAAGGATGTGGCAATCGGGACGCTTCGATACA
TTTGGAAGCAAGCCGGTCTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12281.89 Da        Isoelectric Point: 4.4128

>AT194726 WP_015953962.1 NZ_CP070951:c554650-554315 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194726 NZ_CP093940:c123414-122968 [Pseudomonas sp. I3-I5]
ATGAAATACCCAGTTGTTTTGCACAAAGATGAAGACTCCGACTACGGAGTGACAGTTCCAGACGTACCGGGCTGCTTCTC
TGCTGGCGAAACGATCGATGAAGCATTGAGCGAAGTCCAACTCGCGTTAGAGATGCACTTTGAAGGTTTGGTAGAAGATG
GAGAGCCTCTGCCGCAACCTCAAAATATCGAAATCCATAGGAAGGACCCGGCTTATGCCGATGGCATCTGGGGTTTCGTA
GAATTCGACGTCACCCCCTACTATGGGAAGTCGGTTCGCTTTAACGCGTCTTTACCAGAAGGTCTCCTCAAACGCATAGA
TGATCGCGTGAAAGAAGACAGCGCTTATGCGTCCAGATCAGGATTTTTAGCCGCGGCGGCGCTGCATGAGCTGGCGAAAA
ACTTAAACGAAGGCATAGACGCTTTAAAAACCAGAAAGACAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB B7LMV4

References