Detailed information of TA system TA194721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3987935..3988347
• Replicon: chromosome
• Accession: NZ_CP070930
• Organism: Escherichia coli strain 30COLEC

Toxin (Protein)

• Gene name: symE
• Uniprot ID: B1IS82
• Locus tag: JR883_RS19125
• Protein ID: WP_000132625.1
• Coordinates: 3988006..3988347 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3987935..3988011 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR883_RS19115	3984553..3986022	+	1470	WP_061359977.1	type I restriction-modification system subunit M	-
JR883_RS19120	3986022..3987785	+	1764	WP_096973077.1	restriction endonuclease subunit S	-
-	3987935..3988011	-	77	NuclAT_15	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_15	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_15	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_15	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_16	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_16	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_16	-	Antitoxin
-	3987935..3988011	-	77	NuclAT_16	-	Antitoxin
JR883_RS19125	3988006..3988347	+	342	WP_000132625.1	endoribonuclease SymE	Toxin
JR883_RS19130	3988394..3990487	-	2094	WP_205899517.1	DUF262 domain-containing protein	-
JR883_RS19135	3990584..3990748	-	165	WP_000394276.1	DUF1127 domain-containing protein	-
JR883_RS19140	3990925..3992337	+	1413	WP_000199353.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12270.10 Da
• Isoelectric Point: 8.4952

>T194721 WP_000132625.1 NZ_CP070930:3988006-3988347 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVVVKVMEGCIVLTAQPAAAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T194721 NZ_CP093935:c2183874-2183665 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT194721 NZ_CP070930:c3988011-3987935 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: B1IS82

Antitoxin