Detailed information of TA system TA194719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3625063..3625757
• Replicon: chromosome
• Accession: NZ_CP070930
• Organism: Escherichia coli strain 30COLEC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: JR883_RS17405
• Protein ID: WP_151281599.1
• Coordinates: 3625063..3625461 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JR883_RS17410
• Protein ID: WP_000554757.1
• Coordinates: 3625464..3625757 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR883_RS17375	3620063..3621307	-	1245	WP_000189541.1	esterase FrsA	-
-	3620723..3620803	-	81	NuclAT_13	-	-
-	3620723..3620803	-	81	NuclAT_13	-	-
-	3620723..3620803	-	81	NuclAT_13	-	-
-	3620723..3620803	-	81	NuclAT_13	-	-
JR883_RS17380	3621399..3621857	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JR883_RS17385	3622118..3623575	+	1458	WP_001293013.1	cytosol nonspecific dipeptidase	-
JR883_RS17390	3623632..3624153	-	522	Protein_3396	peptide chain release factor H	-
JR883_RS17395	3624152..3624355	-	204	Protein_3397	RNA ligase RtcB family protein	-
JR883_RS17400	3624601..3625053	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JR883_RS17405	3625063..3625461	-	399	WP_151281599.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JR883_RS17410	3625464..3625757	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JR883_RS17415	3625809..3626864	-	1056	WP_001226164.1	DNA polymerase IV	-
JR883_RS17420	3626935..3627858	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
JR883_RS17425	3627861..3628724	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
JR883_RS17430	3628737..3629453	-	717	WP_205899504.1	FliA/WhiG family RNA polymerase sigma factor	-
JR883_RS17435	3629473..3629940	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15516.87 Da
• Isoelectric Point: 8.5471

>T194719 WP_151281599.1 NZ_CP070930:c3625461-3625063 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYFQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T194719 NZ_CP093935:c2070494-2070318 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT194719 WP_000554757.1 NZ_CP070930:c3625757-3625464 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT194719 NZ_CP093935:2070195-2070250 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Antitoxin

• AlphaFold DB: S1FJN6