Detailed information of TA system TA194702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 661751..662478
• Replicon: chromosome
• Accession: NZ_CP070930
• Organism: Escherichia coli strain 30COLEC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: JR883_RS03165
• Protein ID: WP_000550192.1
• Coordinates: 661751..662065 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JR883_RS03170
• Protein ID: WP_000560266.1
• Coordinates: 662062..662478 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR883_RS03145	657927..658922	-	996	WP_205899548.1	Gfo/Idh/MocA family oxidoreductase	-
JR883_RS03150	658983..659666	-	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
JR883_RS03155	659743..660246	-	504	WP_001295542.1	M48 family metallopeptidase	-
JR883_RS03160	660331..661467	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
JR883_RS03165	661751..662065	+	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
JR883_RS03170	662062..662478	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
JR883_RS03175	662523..664541	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
JR883_RS03180	664967..667318	-	2352	WP_000695486.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T194702 WP_000550192.1 NZ_CP070930:661751-662065 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T194702 NZ_CP093933:c833178-832990 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT194702 WP_000560266.1 NZ_CP070930:662062-662478 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT194702 NZ_CP093933:c833467-833207 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: S1EX98