Detailed information of TA system TA194701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 613161..613960
• Replicon: chromosome
• Accession: NZ_CP070930
• Organism: Escherichia coli strain 30COLEC

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: JR883_RS02925
• Protein ID: WP_000347251.1
• Coordinates: 613161..613625 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: JR883_RS02930
• Protein ID: WP_012304834.1
• Coordinates: 613625..613960 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR883_RS02895	608162..608596	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JR883_RS02900	608614..609492	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JR883_RS02905	609482..610261	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JR883_RS02910	610272..610745	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JR883_RS02915	610768..612048	-	1281	WP_205899547.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JR883_RS02920	612297..613106	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JR883_RS02925	613161..613625	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JR883_RS02930	613625..613960	-	336	WP_012304834.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JR883_RS02935	614109..615680	-	1572	WP_001273760.1	galactarate dehydratase	-
JR883_RS02940	616055..617389	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JR883_RS02945	617405..618175	+	771	WP_001058236.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T194701 WP_000347251.1 NZ_CP070930:c613625-613161 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T194701 NZ_CP093930:c2556201-2555935 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.95 Da
• Isoelectric Point: 4.6890

>AT194701 WP_012304834.1 NZ_CP070930:c613960-613625 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT194701 NZ_CP093930:c2556458-2556201 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20