Detailed information of TA system TA194687

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104503..104929
• Replicon: plasmid p40EC-1
• Accession: NZ_CP070921
• Organism: Escherichia coli strain 40EC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JR909_RS23595
• Protein ID: WP_001312861.1
• Coordinates: 104503..104661 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104705..104929 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR909_RS23560	99548..99775	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JR909_RS23565	99869..100555	-	687	WP_000332484.1	PAS domain-containing protein	-
JR909_RS23570	100746..101129	-	384	WP_000124981.1	relaxosome protein TraM	-
JR909_RS23575	101406..102053	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
JR909_RS23580	102350..103171	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JR909_RS23585	103294..103581	-	288	WP_000107535.1	hypothetical protein	-
JR909_RS23590	103606..103812	-	207	WP_000547939.1	hypothetical protein	-
JR909_RS23595	104503..104661	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	104705..104929	-	225	NuclAT_0	-	Antitoxin
-	104705..104929	-	225	NuclAT_0	-	Antitoxin
-	104705..104929	-	225	NuclAT_0	-	Antitoxin
-	104705..104929	-	225	NuclAT_0	-	Antitoxin
JR909_RS23600	104741..104929	+	189	WP_001299721.1	hypothetical protein	-
JR909_RS23605	104941..105660	-	720	WP_205899292.1	plasmid SOS inhibition protein A	-
JR909_RS23610	105657..106091	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
JR909_RS23615	106146..108104	-	1959	Protein_106	ParB/RepB/Spo0J family partition protein	-
JR909_RS23620	108163..108396	-	234	WP_000006004.1	DUF905 domain-containing protein	-
JR909_RS23625	108452..108979	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
JR909_RS23630	109449..109721	-	273	WP_072133095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..131966	131966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T194687 WP_001312861.1 NZ_CP070921:c104661-104503 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T194687 NZ_CP093927:c90238-89981 [Enterococcus hirae]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGCTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT194687 NZ_CP070921:c104929-104705 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin