194666

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 591495..592294 Replicon chromosome
Accession NZ_CP070920
Organism Escherichia coli strain 40EC

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JR909_RS02860 Protein ID WP_000347267.1
Coordinates 591495..591959 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JR909_RS02865 Protein ID WP_001307405.1
Coordinates 591959..592294 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR909_RS02830 586496..586930 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JR909_RS02835 586948..587826 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JR909_RS02840 587816..588595 - 780 WP_001543177.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JR909_RS02845 588606..589079 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JR909_RS02850 589102..590382 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JR909_RS02855 590631..591440 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JR909_RS02860 591495..591959 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JR909_RS02865 591959..592294 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JR909_RS02870 592443..594014 - 1572 WP_001273741.1 galactarate dehydratase -
JR909_RS02875 594389..595723 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JR909_RS02880 595739..596509 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 591495..602950 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T194666 WP_000347267.1 NZ_CP070920:c591959-591495 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T194666 NZ_CP093919:1077630-1077965 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT194666 WP_001307405.1 NZ_CP070920:c592294-591959 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194666 NZ_CP093919:1077382-1077630 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References