Detailed information of TA system TA194661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3668581..3669275
• Replicon: chromosome
• Accession: NZ_CP070914
• Organism: Escherichia coli strain 65COLEC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JR892_RS17745
• Protein ID: WP_001263493.1
• Coordinates: 3668581..3668979 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JR892_RS17750
• Protein ID: WP_000554757.1
• Coordinates: 3668982..3669275 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR892_RS17715	3663581..3664825	-	1245	WP_000189541.1	esterase FrsA	-
-	3664241..3664321	-	81	NuclAT_13	-	-
-	3664241..3664321	-	81	NuclAT_13	-	-
-	3664241..3664321	-	81	NuclAT_13	-	-
-	3664241..3664321	-	81	NuclAT_13	-	-
JR892_RS17720	3664917..3665375	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JR892_RS17725	3665636..3667093	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JR892_RS17730	3667150..3667671	-	522	Protein_3468	peptide chain release factor H	-
JR892_RS17735	3667670..3667873	-	204	Protein_3469	RNA ligase RtcB family protein	-
JR892_RS17740	3668119..3668571	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JR892_RS17745	3668581..3668979	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JR892_RS17750	3668982..3669275	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JR892_RS17755	3669327..3670382	-	1056	WP_085452615.1	DNA polymerase IV	-
JR892_RS17760	3670453..3671238	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
JR892_RS17765	3671210..3672922	+	1713	Protein_3475	flagellar biosynthesis protein FlhA	-
JR892_RS17770	3673138..3673635	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3639767..3674437	34670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T194661 WP_001263493.1 NZ_CP070914:c3668979-3668581 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T194661 NZ_CP093919:c611913-611449 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT194661 WP_000554757.1 NZ_CP070914:c3669275-3668982 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT194661 NZ_CP093919:c612248-611913 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6