Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1811036..1811538 Replicon chromosome
Accession NZ_CP070914
Organism Escherichia coli strain 65COLEC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JR892_RS08530 Protein ID WP_000767829.1
Coordinates 1811284..1811538 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JR892_RS08525 Protein ID WP_001259255.1
Coordinates 1811036..1811287 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR892_RS08500 1806214..1807281 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JR892_RS08505 1807281..1808351 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JR892_RS08510 1808348..1809652 - 1305 WP_004991367.1 histidinol dehydrogenase -
JR892_RS08515 1809658..1810557 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
JR892_RS08520 1810703..1810753 - 51 WP_001364200.1 his operon leader peptide -
JR892_RS08525 1811036..1811287 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JR892_RS08530 1811284..1811538 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JR892_RS08535 1811621..1812445 + 825 WP_000754758.1 SDR family oxidoreductase -
JR892_RS08540 1812491..1813420 + 930 WP_000803351.1 LysR family transcriptional regulator -
JR892_RS08545 1813635..1813697 + 63 WP_010723108.1 membrane protein YoeI -
JR892_RS08550 1813687..1815045 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JR892_RS08555 1815224..1816282 + 1059 WP_088537866.1 transport protein YeeE -
JR892_RS08560 1816296..1816523 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T194648 WP_000767829.1 NZ_CP070914:1811284-1811538 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T194648 NZ_CP093914:c285832-285545 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTGGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACTGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGGGAGCAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTTAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCCGCCGGGGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT194648 WP_001259255.1 NZ_CP070914:1811036-1811287 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT194648 NZ_CP093914:c286141-285839 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAAGATCGGCGAGCAAGGACCATTATCGC
GTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAATTGCTGAGCAATGTATCCCAAGGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References