Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 660619..661346 Replicon chromosome
Accession NZ_CP070914
Organism Escherichia coli strain 65COLEC

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JR892_RS03200 Protein ID WP_000550189.1
Coordinates 660619..660933 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JR892_RS03205 Protein ID WP_000560266.1
Coordinates 660930..661346 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR892_RS03180 656777..657763 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
JR892_RS03185 657842..658534 - 693 WP_001411307.1 vancomycin high temperature exclusion protein -
JR892_RS03190 658611..659114 - 504 WP_001401153.1 M48 family metallopeptidase -
JR892_RS03195 659199..660335 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JR892_RS03200 660619..660933 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JR892_RS03205 660930..661346 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JR892_RS03210 661391..663409 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JR892_RS03215 663835..666186 - 2352 WP_032181363.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T194644 WP_000550189.1 NZ_CP070914:660619-660933 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T194644 NZ_CP093913:c991198-990848 [Aggregatibacter actinomycetemcomitans]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT194644 WP_000560266.1 NZ_CP070914:660930-661346 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT194644 NZ_CP093913:c990851-990552 [Aggregatibacter actinomycetemcomitans]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCCTCAAACGTTACATCGAAGCAATGGGC
GGCAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGTTTTTTCAAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References