Detailed information of TA system TA194643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 617386..618185
• Replicon: chromosome
• Accession: NZ_CP070914
• Organism: Escherichia coli strain 65COLEC

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JR892_RS02980
• Protein ID: WP_000347273.1
• Coordinates: 617386..617850 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: JR892_RS02985
• Protein ID: WP_021547164.1
• Coordinates: 617850..618185 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR892_RS02950	612387..612821	-	435	WP_000948814.1	PTS sugar transporter subunit IIA	-
JR892_RS02955	612839..613717	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JR892_RS02960	613707..614486	-	780	WP_077492473.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JR892_RS02965	614497..614970	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JR892_RS02970	614993..616273	-	1281	WP_205900159.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JR892_RS02975	616522..617331	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JR892_RS02980	617386..617850	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JR892_RS02985	617850..618185	-	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JR892_RS02990	618334..619905	-	1572	WP_001753084.1	galactarate dehydratase	-
JR892_RS02995	620280..621614	+	1335	WP_077492475.1	galactarate/glucarate/glycerate transporter GarP	-
JR892_RS03000	621630..622400	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T194643 WP_000347273.1 NZ_CP070914:c617850-617386 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T194643 NZ_CP093913:948506-948688 [Aggregatibacter actinomycetemcomitans]
ATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAA
GGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCA
AAAAAGACTTAAACTTAAAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT194643 WP_021547164.1 NZ_CP070914:c618185-617850 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT194643 NZ_CP093913:948740-949159 [Aggregatibacter actinomycetemcomitans]
ATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGC
AAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTCGAATTTTATTTTGATG
ATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAA
GTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGAAATGGCGCGCCGTCTTGATGTTCACATGCCGCA
AGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAA
TTAATTTATCGGTTCATTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39