Detailed information of TA system TA194637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4684525..4685127
• Replicon: chromosome
• Accession: NZ_CP070906
• Organism: Escherichia coli strain 88COLEC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JR901_RS22170
• Protein ID: WP_000897305.1
• Coordinates: 4684816..4685127 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JR901_RS22165
• Protein ID: WP_000356397.1
• Coordinates: 4684525..4684815 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR901_RS22140	4680469..4681371	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JR901_RS22145	4681368..4682003	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JR901_RS22150	4682000..4682929	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JR901_RS22155	4683259..4683501	-	243	WP_001087409.1	hypothetical protein	-
JR901_RS22160	4683721..4683939	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
JR901_RS22165	4684525..4684815	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JR901_RS22170	4684816..4685127	-	312	WP_000897305.1	hypothetical protein	Toxin
JR901_RS22175	4685356..4686264	+	909	WP_001162704.1	alpha/beta hydrolase	-
JR901_RS22180	4686328..4687269	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JR901_RS22185	4687314..4687751	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JR901_RS22190	4687748..4688620	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JR901_RS22195	4688614..4689213	-	600	WP_001295269.1	glucose-1-phosphatase	-
JR901_RS22200	4689312..4690097	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T194637 WP_000897305.1 NZ_CP070906:c4685127-4684816 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T194637 NZ_CP093913:c19153-18971 [Aggregatibacter actinomycetemcomitans]
ATGAAATACAGTGAATTCTTGCGATACTTGCTTGATCAAGGCTGCAAAGTCGAATCAACCAAGCGCGGAAGTCATCGCAA
GGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCA
AAAAAGACTTAAACTTAAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT194637 WP_000356397.1 NZ_CP070906:c4684815-4684525 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT194637 NZ_CP093913:c18919-18500 [Aggregatibacter actinomycetemcomitans]
ATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGT
AAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACAGCCCTTGAATTTTATTTTGATG
ATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCACTTTCCTTATTGCGTTCACTAAAA
GTCTTCTTACTCAATGAAATGCTCACACAAGGTGTGCGAAAAGCTGAAATGGCGCGCCGTCTTGATGTTCACATGCCGCA
AGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAA
TTAATTTATCGGTTCATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7